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Targeted Allele Detail
Symbol: Kcnj5tm1Clph
Name: potassium inwardly-rectifying channel, subfamily J, member 5; targeted mutation 1, David E Clapham
MGI ID: MGI:2181760
Synonyms: GIRK4 (-)
Gene: Kcnj5  Location: Chr9:32314783-32344237 bp, - strand  Genetic Position: Chr9, 17.65 cM
Germline Transmission:  Earliest citation of germline transmission: J:45533
Parent Cell Line:  Not Specified (ES Cell)
Strain of Origin:  129X1/SvJ
Allele Type:    Targeted (Null/knockout)
Mutations:    Insertion, Intragenic deletion
Mutation detailsA neomycin selection cassette replaced exon 1, which encodes the amino and carboxyl termini, both transmembrane domains, and the putative pore-lining sequence. Northern blot analysis of total atrial RNA showed an absence of normal transcript in homozygous mutant mice. mRNA levels of other K+ inwardly-rectifying channels, were shown to be unaffected by the targeting event by rehybridization of the Northern blot using Kcnj3-, Kcnj6- and Kcnj9-specific probes. Western blot analysis of protein extracts derived from atrial cell membranes showed that the targeted Kcnj5 protein product was absent, and levels of both glycosylated and unglycosylated forms of Kcnj3 were significantly reduced in homozygous mutant mice. (J:45533)
View phenotypes for all genotypes (concatenated display).
Find Mice (IMSR)
Mouse strains and cell lines available from the International Mouse Strain Resource (IMSR)
Carrying this Mutation:  Mouse Strains: 0 strains available      Cell Lines: 0 lines available
Carrying any Kcnj5 Mutation:  13 strains or lines available
Original:  J:45533 Wickman K, et al., Abnormal heart rate regulation in GIRK4 knockout mice. Neuron. 1998 Jan;20(1):103-14
All:  13 reference(s)

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MGI 6.06
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