Slc12a2^tm2Bhk
Targeted Allele Detail

Summary

• Symbol: Slc12a2^tm2Bhk
• Name: solute carrier family 12, member 2; targeted mutation 2, Beverly H Koller
• MGI ID: MGI:2181638
• Synonyms: Slc12a2^-, Slc12a2^delta506-621
• Gene: Slc12a2 Location: Chr18:58011750-58079893 bp, + strand Genetic Position: Chr18, 32.15 cM
• Alliance: Slc12a2^tm2Bhk page

Mutation origin

• Germline Transmission: Earliest citation of germline transmission: J:60671
• Parent Cell Line: E14TG2a (ES Cell)
• Strain of Origin: 129P2/OlaHsd

Mutation description

• Allele Type: Targeted (Null/knockout)
• Mutations: Insertion, Intragenic deletion
• Mutation details: Exons 9, 10, and 11, encoding transmembrane domains 7 and 8 (amino acids 506 through 621), were replaced with a neomycin selection cassette. Northern blot analysis of total RNA extracted from salivary glands showed an absence of normal transcript in homozygous mutant mice, though low levels of a transcript containing the neo transgene were detected. This mutant transcript was not expected to encode a functional protein. (J:60671)

Expression

In Structures Affected by this Mutation:

7 anatomical structure(s)

Find Mice (IMSR)

• Mouse strains and cell lines available from the International Mouse Strain Resource (IMSR)
• Carrying this Mutation: Mouse Strains: 0 strains available Cell Lines: 0 lines available
• Carrying any Slc12a2 Mutation: 58 strains or lines available

References

• Original: J:60671 Pace AJ, et al., Failure of spermatogenesis in mouse lines deficient in the Na(+)-K(+)-2Cl(-) cotransporter. J Clin Invest. 2000 Feb;105(4):441-50
• All: 10 reference(s)