Smad6^tm1Glvn
Targeted Allele Detail

Summary

• Symbol: Smad6^tm1Glvn
• Name: SMAD family member 6; targeted mutation 1, Katherine M Galvin
• MGI ID: MGI:2181630
• Synonyms: Madh6^-
• Gene: Smad6 Location: Chr9:63860358-63929341 bp, - strand Genetic Position: Chr9, 34.4 cM, cytoband D
• Alliance: Smad6^tm1Glvn page

Mutation origin

• Germline Transmission: Earliest citation of germline transmission: J:60214
• Parent Cell Line: MPI-12D (ES Cell)
• Strain of Origin: 129S6/SvEvTac

Mutation description

• Allele Type: Targeted (Null/knockout, Reporter)
• Mutations: Insertion, Intragenic deletion
• Mutation details: A cassette containing an NLS-lacZ gene adjoined to a neo gene replaced the 5' terminus of the exon encoding the MH2 domain, which has been implicated as the major effector domain. Northern blot analyses of lung tissue detected the lacZ fusion transcript, lacking the targeted MH2 domain, in mutant mice. Comparison of X-gal staining and in situ hybridization results showed that the expression pattern of beta-galactosidase resembled that of the endogenous locus in wild-type mice, with the exception of upregulation in the epi- and endocardial cells of the right atrium in homozygous mutant mice. (J:60214)

Expression

In Mice Carrying this Mutation:	45 assay results
In Structures Affected by this Mutation:	12 anatomical structure(s)

Find Mice (IMSR)

• Mouse strains and cell lines available from the International Mouse Strain Resource (IMSR)
• Carrying this Mutation: Mouse Strains: 0 strains available Cell Lines: 0 lines available
• Carrying any Smad6 Mutation: 23 strains or lines available

References

• Original: J:60214 Galvin KM, et al., A role for smad6 in development and homeostasis of the cardiovascular system. Nat Genet. 2000 Feb;24(2):171-4
• All: 4 reference(s)