Itgb4tm1Fgg
Targeted Allele Detail
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Symbol: |
Itgb4tm1Fgg |
Name: |
integrin beta 4; targeted mutation 1, Filippo G Giancotti |
MGI ID: |
MGI:2181069 |
Synonyms: |
Beta4TL |
Gene: |
Itgb4 Location: Chr11:115865556-115899238 bp, + strand Genetic Position: Chr11, 80.91 cM
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Alliance: |
Itgb4tm1Fgg page
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Allele Type: |
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Targeted (Null/knockout) |
Mutation: |
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Insertion
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Mutation details: A cassette containing a stop codon, a SV40 polyadenylation site, and a neomycin resistance gene was inserted immediately downstream of the exon encoding the transmembrane domain (upstream of sequence encoding the cytoplasmic domain). Expression analyses showed the presence of truncated transcript and protein in mutant mice. While Northern blot analysis detected increased levels of mutant transcript, Western blot analysis showed protein levels to be comprable to wild-type. FACS analysis confirmed that mutant protein was correctly expressed on the cell surface.
(J:48924)
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View phenotypes and curated references for all genotypes (concatenated display).
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Mouse strains and cell lines
available from the International Mouse Strain Resource
(IMSR) |
Carrying this Mutation: |
Mouse Strains: 0 strains available
Cell Lines: 0 lines available
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Carrying any Itgb4 Mutation: |
107 strains or lines available
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Original: |
J:48924 Murgia C, et al., Cell cycle and adhesion defects in mice carrying a targeted deletion of the integrin beta4 cytoplasmic domain. EMBO J. 1998 Jul 15;17(14):3940-51 |
All: |
1 reference(s) |
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