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Targeted Allele Detail
Symbol: Matktm1Hua
Name: megakaryocyte-associated tyrosine kinase; targeted mutation 1, Hua Gu
MGI ID: MGI:2180863
Synonyms: chk-
Gene: Matk  Location: Chr10:81252935-81263365 bp, + strand  Genetic Position: Chr10, 39.72 cM, cytoband C1
Germline Transmission:  Earliest citation of germline transmission: J:47538
Parent Cell Line:  E14.1 (ES Cell)
Strain of Origin:  129P2/OlaHsd
Allele Type:    Targeted (Null/knockout)
Mutation:    Intragenic deletion
Mutation detailsA construct was created in which a floxed neomycin cassette was inserted immediately upstream of the exons encoding the catalytic tyrosine kinase domain. An additional loxP site was inserted at the 3' terminal of this region. Transient transfection of ES cells with plasmids expressing cre-recombinase generated an allele which lacked both the inserted neomycin gene and the catalytic domain encoding exon. Northern blot analyses using various probes showed that a truncated transcript lacking the catalytic domain was produced in homozygous mutant mice. Additionally, absence of the tyrosine kinase domain encoding region was confirmed by RT-PCR. (J:47538)
View phenotypes and curated references for all genotypes (concatenated display).
Find Mice (IMSR)
Mouse strains and cell lines available from the International Mouse Strain Resource (IMSR)
Carrying this Mutation:  Mouse Strains: 0 strains available      Cell Lines: 0 lines available
Carrying any Matk Mutation:  21 strains or lines available
Original:  J:47538 Samokhvalov I, et al., Mice lacking a functional chk gene have no apparent defects in the hematopoietic system. Biochem Mol Biol Int. 1997 Sep;43(1):115-22
All:  1 reference(s)

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