Matk^tm1Hua
Targeted Allele Detail

Summary

• Symbol: Matk^tm1Hua
• Name: megakaryocyte-associated tyrosine kinase; targeted mutation 1, Hua Gu
• MGI ID: MGI:2180863
• Synonyms: chk^-
• Gene: Matk Location: Chr10:81088769-81098819 bp, + strand Genetic Position: Chr10, 39.72 cM, cytoband C1
• Alliance: Matk^tm1Hua page

Mutation origin

• Germline Transmission: Earliest citation of germline transmission: J:47538
• Parent Cell Line: E14.1 (ES Cell)
• Strain of Origin: 129P2/OlaHsd

Mutation description

• Allele Type: Targeted (Null/knockout)
• Mutation: Intragenic deletion
• Mutation details: A construct was created in which a floxed neomycin cassette was inserted immediately upstream of the exons encoding the catalytic tyrosine kinase domain. An additional loxP site was inserted at the 3' terminal of this region. Transient transfection of ES cells with plasmids expressing cre-recombinase generated an allele which lacked both the inserted neomycin gene and the catalytic domain encoding exon. Northern blot analyses using various probes showed that a truncated transcript lacking the catalytic domain was produced in homozygous mutant mice. Additionally, absence of the tyrosine kinase domain encoding region was confirmed by RT-PCR. (J:47538)

Find Mice (IMSR)

• Mouse strains and cell lines available from the International Mouse Strain Resource (IMSR)
• Carrying this Mutation: Mouse Strains: 0 strains available Cell Lines: 0 lines available
• Carrying any Matk Mutation: 30 strains or lines available

References

• Original: J:47538 Samokhvalov I, et al., Mice lacking a functional chk gene have no apparent defects in the hematopoietic system. Biochem Mol Biol Int. 1997 Sep;43(1):115-22
• All: 1 reference(s)