Ceacam1^tm1Bcn
Targeted Allele Detail

Summary

• Symbol: Ceacam1^tm1Bcn
• Name: CEA cell adhesion molecule 1; targeted mutation 1, Nicole Beauchemin
• MGI ID: MGI:2180785
• Synonyms: Ceacam1aDelta4D, p
• Gene: Ceacam1 Location: Chr7:25161132-25177028 bp, - strand Genetic Position: Chr7, 13.84 cM
• Alliance: Ceacam1^tm1Bcn page

Mutation origin

• Germline Transmission: Earliest citation of germline transmission: J:70815
• Parent Cell Line: R1 (ES Cell)
• Strain of Origin: (129X1/SvJ x 129S1/Sv)F1-Kitl⁺

Mutation description

• Allele Type: Targeted (Null/knockout)
• Mutation: Insertion
• Mutation details: An allele with both hypo- and hypermorphic characteristics was generated by the insertion of a neomycin selection cassette in to intron 2. While the targeting vector was designed to incorporate engineered stop codons into exon 2 and thereby completely ablate expression of the four known isoforms, aberrant recombination resulted in the exclusion of the stop codons. Western blot analysis of various tissues showed low levels (less than 10% of wild-type) of the two D1-4 isoforms and increased levels of the two D1,4 isoforms that were double those of wild-type. (J:70815)

Find Mice (IMSR)

• Mouse strains and cell lines available from the International Mouse Strain Resource (IMSR)
• Carrying this Mutation: Mouse Strains: 0 strains available Cell Lines: 0 lines available
• Carrying any Ceacam1 Mutation: 45 strains or lines available

References

• Original: J:70815 Blau DM, et al., Targeted disruption of the Ceacam1 (MHVR) gene leads to reduced susceptibility of mice to mouse hepatitis virus infection. J Virol. 2001 Sep;75(17):8173-86
• All: 6 reference(s)