Cd69^tm1Fsm
Targeted Allele Detail

Summary

• Symbol: Cd69^tm1Fsm
• Name: CD69 antigen; targeted mutation 1, Francisco Sanchez-Madrid
• MGI ID: MGI:2180772
• Synonyms: CD69 (-)
• Gene: Cd69 Location: Chr6:129244287-129252332 bp, - strand Genetic Position: Chr6, 63.21 cM
• Alliance: Cd69^tm1Fsm page

Mutation origin

• Germline Transmission: Earliest citation of germline transmission: J:61329
• Parent Cell Line: R1 (ES Cell)
• Strain of Origin: (129X1/SvJ x 129S1/Sv)F1-Kitl⁺

Mutation description

• Allele Type: Targeted (Null/knockout)
• Mutations: Insertion, Intragenic deletion
• Mutation details: A neomycin selection gene was inserted to delete all of exons 2, 3, and 4, and the translated region of exon 5. Flow cytometric analysis of thymocytes stained with a monoclonal antibody revealed a lack of surface expression of the encoded protein in homozygous mutant mice. Additional analysis via flow cytometry showed an absence of protein expression in T cells derived from the kidney, spleen, and peripheral blood when activated with PMA. (J:61329)

Expression

In Structures Affected by this Mutation:

1 anatomical structure(s)

Find Mice (IMSR)

• Mouse strains and cell lines available from the International Mouse Strain Resource (IMSR)
• Carrying this Mutation: Mouse Strains: 0 strains available Cell Lines: 0 lines available
• Carrying any Cd69 Mutation: 21 strains or lines available

References

• Original: J:61329 Lauzurica P, et al., Phenotypic and functional characteristics of hematopoietic cell lineages in CD69-deficient mice. Blood. 2000 Apr 1;95(7):2312-20
• All: 15 reference(s)