Cd34^tm1Szk
Targeted Allele Detail

Summary

• Symbol: Cd34^tm1Szk
• Name: CD34 antigen; targeted mutation 1, Akira Suzuki
• MGI ID: MGI:2180755
• Gene: Cd34 Location: Chr1:194621239-194643587 bp, + strand Genetic Position: Chr1, 98.38 cM
• Alliance: Cd34^tm1Szk page

Mutation origin

• Germline Transmission: Earliest citation of germline transmission: J:32799
• Parent Cell Line: E14 (ES Cell)
• Strain of Origin: 129P2/OlaHsd

Mutation description

• Allele Type: Targeted (Null/knockout)
• Mutations: Insertion, Intragenic deletion
• Mutation details: The insertion of neomycin selection cassette deleted a 0.72-kb region including portions of exon 7 and exon 8 and encoding the entire transmembrane domain (amino acids 270 through 326). Staining of bone marrow cells isolated from homozygous mutant mice determined an absence of protein surface expression. Immunohistochemical staining and Western blot analysis confirmed a lack of protein in the lymph nodes of homozygous mutant mice. (J:32799)

Expression

In Structures Affected by this Mutation:

4 anatomical structure(s)

Find Mice (IMSR)

• Mouse strains and cell lines available from the International Mouse Strain Resource (IMSR)
• Carrying this Mutation: Mouse Strains: 1 strain available Cell Lines: 0 lines available
• Carrying any Cd34 Mutation: 31 strains or lines available

References

• Original: J:32799 Suzuki A, et al., CD34-deficient mice have reduced eosinophil accumulation after allergen exposure and show a novel crossreactive 90-kD protein. Blood. 1996 May 1;87(9):3550-62
• All: 15 reference(s)