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Acrtm1Tba
Targeted Allele Detail
Nomenclature
Symbol: Acrtm1Tba
Name: acrosin prepropeptide; targeted mutation 1, Tadashi Baba
MGI ID: MGI:2180087
Gene: Acr  Location: Chr15:89568326-89574585 bp, + strand  Genetic Position: Chr15, 44.96 cM, cytoband E-F
Mutation
origin
Germline Transmission:  Earliest citation of germline transmission: J:21944
Parent Cell Line:  A3-1 (ES Cell)
Strain of Origin:  129X1/SvJ
Mutation
description
Allele Type:    Targeted (Null/knockout)
Mutations:    Insertion, Intragenic deletion
 
Mutation detailsA neomycin selection cassette was used to replace a portion of exon 2 and all of exon 3. The deleted region encoded His70 and Asp124, which are required for serine protease activity, as well as 5 Cys residues involved in disulfide bonds. Northern analysis using a cDNA fragment containing exons 2 and 3 showed an absence of mRNA in extracts isolated from the testes of homozygous mutant mice. Protease activity in cauda epididymal sperm was assayed and found to be absent in homozygous animals. (J:21944)
Phenotypes
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Find Mice (IMSR)
Mouse strains and cell lines available from the International Mouse Strain Resource (IMSR)
Carrying this Mutation:  Mouse Strains: 1 strain available      Cell Lines: 0 lines available
Carrying any Acr Mutation:  12 strains or lines available
References
Original:  J:21944 Baba T, et al., Sperm from mice carrying a targeted mutation of the acrosin gene can penetrate the oocyte zona pellucida and effect fertilization. J Biol Chem. 1994 Dec 16;269(50):31845-9
All:  3 reference(s)

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Mouse Genome Database (MGD), Gene Expression Database (GXD), Mouse Tumor Biology (MTB), Gene Ontology (GO), MouseCyc
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last database update
11/29/2016
MGI 6.06
The Jackson Laboratory