Nfkb1^tm1Brv
Targeted Allele Detail

Summary

• Symbol: Nfkb1^tm1Brv
• Name: nuclear factor of kappa light polypeptide gene enhancer in B cells 1, p105; targeted mutation 1, Rodrigo Bravo
• MGI ID: MGI:2179707
• Synonyms: NF-kappaB KO, Nfkb1^deltaCT, p105 -, p50
• Gene: Nfkb1 Location: Chr3:135290416-135397308 bp, - strand Genetic Position: Chr3, 62.82 cM
• Alliance: Nfkb1^tm1Brv page

Mutation origin

• Germline Transmission: Earliest citation of germline transmission: J:46912
• Parent Cell Line: CJ7 (ES Cell)
• Strain of Origin: 129S1/Sv-Oca2⁺ Tyr⁺ Kitl⁺

Mutation description

• Allele Type: Targeted (Null/knockout)
• Mutation: Insertion
• Mutation details: A neomycin resistance cassette was inserted into the gene, disrupting the carboxy terminal domain of the encoded protein. Western blot analysis on lystates derived from thymus of homozygous mice confirmed that the p50 isoform of the protein was expressed, but the p105 precursor was not detectable. (J:46912)

Expression

In Structures Affected by this Mutation:

5 anatomical structure(s)

Find Mice (IMSR)

• Mouse strains and cell lines available from the International Mouse Strain Resource (IMSR)
• Carrying this Mutation: Mouse Strains: 0 strains available Cell Lines: 0 lines available
• Carrying any Nfkb1 Mutation: 99 strains or lines available

References

• Original: J:46912 Ishikawa H, et al., Chronic inflammation and susceptibility to bacterial infections in mice lacking the polypeptide (p)105 precursor (NF-kappaB1) but expressing p50. J Exp Med. 1998 Apr 6;187(7):985-96
• All: 6 reference(s)