Skp2^tm1Kin
Targeted Allele Detail

Summary

• Symbol: Skp2^tm1Kin
• Name: S-phase kinase-associated protein 2; targeted mutation 1, Kei-ichi Nakayama
• MGI ID: MGI:2179051
• Synonyms: Fbxl1^-, Skp2-
• Gene: Skp2 Location: Chr15:9112073-9155512 bp, - strand Genetic Position: Chr15, 3.84 cM, cytoband A2
• Alliance: Skp2^tm1Kin page

Mutation origin

• Germline Transmission: Earliest citation of germline transmission: J:62130
• Parent Cell Line: E14 (ES Cell)
• Strain of Origin: 129P2/OlaHsd

Mutation description

• Allele Type: Targeted (Null/knockout)
• Mutations: Insertion, Intragenic deletion
• Mutation details: Exons 1-4 of this gene were replaced with a PGK-neo-poly(A) cassette via homologous recombination, resulting in the loss of the translation initiation site and F-box domain. RT-PCR verified gene expression was absent in homozygous mutant animals and immunoprecipitation failed to detect full length or truncated protein product. (J:62130)

Expression

In Mice Carrying this Mutation:	83 assay results
In Structures Affected by this Mutation:	2 anatomical structure(s)

Tumor Data

List all tumor models in MMHCdb carrying Skp2^tm1Kin

Find Mice (IMSR)

• Mouse strains and cell lines available from the International Mouse Strain Resource (IMSR)
• Carrying this Mutation: Mouse Strains: 1 strain available Cell Lines: 0 lines available
• Carrying any Skp2 Mutation: 45 strains or lines available

References

• Original: J:62130 Nakayama K, et al., Targeted disruption of Skp2 results in accumulation of cyclin E and p27(Kip1), polyploidy and centrosome overduplication. EMBO J. 2000 May 2;19(9):2069-81
• All: 37 reference(s)