Crhr1^tm1Wrst
Targeted Allele Detail

Summary

• Symbol: Crhr1^tm1Wrst
• Name: corticotropin releasing hormone receptor 1; targeted mutation 1, Wolfgang Wurst
• MGI ID: MGI:2178543
• Synonyms: CRF1 KO, Crhr1 -
• Gene: Crhr1 Location: Chr11:104023681-104066349 bp, + strand Genetic Position: Chr11, 67.77 cM
• Alliance: Crhr1^tm1Wrst page

Mutation origin

• Germline Transmission: Earliest citation of germline transmission: J:76022
• Parent Cell Line: E14.1 (ES Cell)
• Strain of Origin: 129P2/OlaHsd

Mutation description

• Allele Type: Targeted (Null/knockout)
• Mutations: Insertion, Intragenic deletion
• Mutation details: A neomycin resistance cassette replaced a 3.1 kb genomic fragment containing sequences encoding transmembrane domains 4-7, including the G-coupling protein domain and the intracellular cytoplasmic tail. Extracellular field potential measurements in the CA1 region of the hippocampus confirmed disruption of CRH signal transduction: application of CRH increased the population spike amplitudes in CA1 slices of wild-type mice, but not in slices of homozygous mutant mice. (J:76022)

Expression

In Structures Affected by this Mutation:

2 anatomical structure(s)

Find Mice (IMSR)

• Mouse strains and cell lines available from the International Mouse Strain Resource (IMSR)
• Carrying this Mutation: Mouse Strains: 1 strain available Cell Lines: 0 lines available
• Carrying any Crhr1 Mutation: 26 strains or lines available

References

• Original: J:76022 Timpl P, et al., Impaired stress response and reduced anxiety in mice lacking a functional corticotropin-releasing hormone receptor 1. Nat Genet. 1998 Jun;19(2):162-6
• All: 21 reference(s)