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Eif2ak3tm1Dron
Targeted Allele Detail
Nomenclature
Symbol: Eif2ak3tm1Dron
Name: eukaryotic translation initiation factor 2 alpha kinase 3; targeted mutation 1, David Ron
MGI ID: MGI:2177666
Synonyms: perk -
Gene: Eif2ak3  Location: Chr6:70844515-70905245 bp, + strand  Genetic Position: Chr6, 32.09 cM
Mutation
origin
Germline Transmission:  Earliest citation of germline transmission: J:70005
Parent Cell Line:  W4 (ES Cell)
Strain of Origin:  129S6/SvEvTac
Mutation
description
Allele Type:    Targeted (Null/knockout)
Mutations:    Insertion, Intragenic deletion
 
Mutation detailsA neomycin selection cassette replaced 1.6 kb of DNA containing the transmembrane domain. Northern blot analysis demonstrated that at least 10-fold less transcript was produced from this allele that the wild-type. However, western blot analysis on cells derived from homozygous mice confirmed that no detectable protein was expressed from this allele. (J:62257)
Phenotypes
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View phenotypes and curated references for all genotypes (concatenated display).
Disease models
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Expression
In Structures Affected by this Mutation: 5 anatomical structures
Find Mice (IMSR)
Mouse strains and cell lines available from the International Mouse Strain Resource (IMSR)
Carrying this Mutation:  Mouse Strains: 1 strain available      Cell Lines: 0 lines available
Carrying any Eif2ak3 Mutation:  8 strains or lines available
References
Original:  J:62257 Harding HP, et al., Perk is essential for translational regulation and cell survival during the unfolded protein response. Mol Cell. 2000 May;5(5):897-904
All:  27 reference(s)

Contributing Projects:
Mouse Genome Database (MGD), Gene Expression Database (GXD), Mouse Models of Human Cancer database (MMHCdb) (formerly Mouse Tumor Biology (MTB), Gene Ontology (GO)
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last database update
12/03/2019
MGI 6.14
The Jackson Laboratory