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Targeted Allele Detail
Symbol: Brca1tm2Arge
Name: breast cancer 1, early onset; targeted mutation 2, Argiris Efstratiadis
MGI ID: MGI:2177209
Synonyms: Brca1tr
Gene: Brca1  Location: Chr11:101379590-101442781 bp, - strand  Genetic Position: Chr11, 65.18 cM, cytoband D
Germline Transmission:  Earliest citation of germline transmission: J:69187
Parent Cell Line:  Not Specified (ES Cell)
Strain of Origin:  129
Allele Type:    Targeted (Hypomorph)
Mutation:    Insertion
Mutation detailsA loxP-flanked neomycin and thymidine kinase selection cassette was inserted into exon 11. The loxP flanked cassette was removed in ES cells by Cre mediated recombination prior to the production of chimeric mice, leaving a 50 bp insertion containing a loxP site. Northern blot analysis on RNA derived from total RNA of homozygous embryos demonstrated that aberrant transcripts were produced from this allele that either lacked exon 11, or contained exon 11 with the insertion, resulting in a frameshift mutation after the first 924 amino acids. This mutation was made to mimic a truncated protein associated with human breast cancer. (J:69187)
View phenotypes and curated references for all genotypes (concatenated display).
In Structures Affected by this Mutation: 4 anatomical structures
Find Mice (IMSR)
Mouse strains and cell lines available from the International Mouse Strain Resource (IMSR)
Carrying this Mutation:  Mouse Strains: 0 strains available      Cell Lines: 0 lines available
Carrying any Brca1 Mutation:  94 strains or lines available
Original:  J:69187 Ludwig T, et al., Tumorigenesis in mice carrying a truncating Brca1 mutation. Genes Dev. 2001 May 15;15(10):1188-93
All:  4 reference(s)

Contributing Projects:
Mouse Genome Database (MGD), Gene Expression Database (GXD), Mouse Models of Human Cancer database (MMHCdb) (formerly Mouse Tumor Biology (MTB), Gene Ontology (GO)
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last database update
MGI 6.19
The Jackson Laboratory