About   Help   FAQ
Portm1Cbk
Targeted Allele Detail
Nomenclature
Symbol: Portm1Cbk
Name: P450 (cytochrome) oxidoreductase; targeted mutation 1, Charles B Kasper
MGI ID: MGI:2159651
Synonyms: CYPOR-, delta56
Gene: Por  Location: Chr5:135670033-135735326 bp, + strand  Genetic Position: Chr5, 75.34 cM, cytoband G1
Portm1Cbk/Portm1Cbk embryos either exhibit neural tube, cardiac, eye, and limb bud abnormalities or retarded development

Show the 4 phenotype image(s) involving this allele.

Mutation
origin
Germline Transmission:  Earliest citation of germline transmission: J:74666
Parent Cell Line:  R1 (ES Cell)
Strain of Origin:  (129X1/SvJ x 129S1/Sv)F1-Kitl+
Mutation
description
Allele Type:    Targeted (Null/knockout)
Mutations:    Insertion, Intragenic deletion
 
Mutation detailsA neomycin resistance gene replaces 2.5 kb of intron 1 and all but the last 6 bp of exon 2. This allele deletes the sequences encoding the translation start site as well as the membrane-binding domain. The targeting event generated a truncated protein which localized to the cytoplasm and not present in microsomes. Cytochrome c reductase activity in liver microsomes isolated from adult heterozygous mice was 50% that found in wild-type mice. (J:74666)
Phenotypes
Loading...
View phenotypes and curated references for all genotypes (concatenated display).
Find Mice (IMSR)
Mouse strains and cell lines available from the International Mouse Strain Resource (IMSR)
Carrying this Mutation:  Mouse Strains: 0 strains available      Cell Lines: 0 lines available
Carrying any Por Mutation:  67 strains or lines available
Notes
Two mutant cell lines were generated (G1 and H11). While founder mice are indistinguishable H11 contains the desired targeting events while G1 contains Dup(2Csrp2bp-6330439K17Rik)1Bra. This may account for the two types of homozygous mutant types identified.
References
Original:  J:74666 Shen AL, et al., Association of multiple developmental defects and embryonic lethality with loss of microsomal NADPH-cytochrome P450 oxidoreductase. J Biol Chem. 2002 Feb 22;277(8):6536-41
All:  3 reference(s)

Contributing Projects:
Mouse Genome Database (MGD), Gene Expression Database (GXD), Mouse Tumor Biology (MTB), Gene Ontology (GO), MouseCyc
Citing These Resources
Funding Information
Warranty Disclaimer & Copyright Notice
Send questions and comments to User Support.
last database update
10/10/2017
MGI 6.10
The Jackson Laboratory