Vax2^tm1Sba
Targeted Allele Detail

Summary

• Symbol: Vax2^tm1Sba
• Name: ventral anterior homeobox 2; targeted mutation 1, Sandro Banfi
• MGI ID: MGI:2159246
• Synonyms: Vax2^-
• Gene: Vax2 Location: Chr6:83688246-83715295 bp, + strand Genetic Position: Chr6, 35.94 cM
• Alliance: Vax2^tm1Sba page

Mutation origin

• Germline Transmission: Earliest citation of germline transmission: J:74241
• Parent Cell Line: AB2.1 (ES Cell)
• Strain of Origin: 129S7/SvEvBrd-Hprt1^b-m2

Mutation description

• Allele Type: Targeted (Null/knockout)
• Mutations: Insertion, Intragenic deletion
• Mutation details: A PGK-neomycin cassette replaced the second exon, which encodes the first two helices and part of the third helix of the homeodomain. Gene targeting was confirmed by RT-PCR analysis. (J:74241)

Expression

In Mice Carrying this Mutation:

59 assay results 1 RNA-Seq or microarray experiment(s)

Find Mice (IMSR)

• Mouse strains and cell lines available from the International Mouse Strain Resource (IMSR)
• Carrying this Mutation: Mouse Strains: 1 strain available Cell Lines: 0 lines available
• Carrying any Vax2 Mutation: 15 strains or lines available

Notes

Vax2 heterozygotes are fertile, viable and inditiguishable from wild-type. The homozygous mutant embryo shows incomplete closure of the optic fissure leading to coloboma and abnormal projection of the retinal ganglions. Teh mutant was carried on both a mixed 129/CD1 and a mixed 129/C57Bl6 background.

References

• Original: J:74241 Barbieri AM, et al., Vax2 inactivation in mouse determines alteration of the eye dorsal-ventral axis, misrouting of the optic fibres and eye coloboma. Development. 2002 Feb;129(3):805-13
• All: 3 reference(s)