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Cd86tm2Shr
Targeted Allele Detail
Nomenclature
Symbol: Cd86tm2Shr
Name: CD86 antigen; targeted mutation 2, Arlene H Sharpe
MGI ID: MGI:2159105
Synonyms: B7-1/2-, B7-1/B7-2-, B7.1/B7.2-, B7DKO, B7KO, Cd80/86-
Gene: Cd86  Location: Chr16:36603869-36666081 bp, - strand  Genetic Position: Chr16, 25.72 cM, cytoband B5
Mutation
origin
Germline Transmission:  Earliest citation of germline transmission: J:39089
Parent Cell Line:  J1 (ES Cell)
Strain of Origin:  129S4/SvJae
Mutation
description
Allele Type:    Targeted (Null/knockout)
Mutations:    Insertion, Intragenic deletion
 
Mutation detailsTo create a knockout of both Cd80 and Cd86 in mice, an ES cell line in which the Cd80 gene was mutated was subsequently used in a second round of gene targeting. A hygromycin selection cassette replaced an exon encoding the IgV-like domain of the protein and flanking genomic sequences of the Cd86 gene. FACS analysis on splenocytes and LPS-stimulated B cells derived from homozygous mice confirmed that no functional protein was encoded by this allele. (J:39089)
Phenotypes
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View phenotypes for all genotypes (concatenated display).
Disease models
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Find Mice (IMSR)
Mouse strains and cell lines available from the International Mouse Strain Resource (IMSR)
Carrying this Mutation:  Mouse Strains: 2 strains available      Cell Lines: 0 lines available
Carrying any Cd86 Mutation:  12 strains or lines available
References
Original:  J:39089 Borriello F, et al., B7-1 and B7-2 have overlapping, critical roles in immunoglobulin class switching and germinal center formation. Immunity. 1997 Mar;6(3):303-13
All:  99 reference(s)

Contributing Projects:
Mouse Genome Database (MGD), Gene Expression Database (GXD), Mouse Tumor Biology (MTB), Gene Ontology (GO), MouseCyc
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last database update
06/23/2015
MGI 5.22
The Jackson Laboratory