About   Help   FAQ
Targeted Allele Detail
Symbol: Cd86tm2Shr
Name: CD86 antigen; targeted mutation 2, Arlene H Sharpe
MGI ID: MGI:2159105
Synonyms: B7-1/2-, B7-1/B7-2-, B7.1/B7.2-, B7DKO, B7KO, Cd80/86-
Gene: Cd86  Location: Chr16:36603869-36666081 bp, - strand  Genetic Position: Chr16, 25.72 cM, cytoband B5
Germline Transmission:  Earliest citation of germline transmission: J:39089
Parent Cell Line:  J1 (ES Cell)
Strain of Origin:  129S4/SvJae
Allele Type:    Targeted (Null/knockout)
Mutations:    Insertion, Intragenic deletion
Mutation detailsTo create a knockout of both Cd80 and Cd86 in mice, an ES cell line in which the Cd80 gene was mutated was subsequently used in a second round of gene targeting. A hygromycin selection cassette replaced an exon encoding the IgV-like domain of the protein and flanking genomic sequences of the Cd86 gene. FACS analysis on splenocytes and LPS-stimulated B cells derived from homozygous mice confirmed that no functional protein was encoded by this allele. (J:39089)
View phenotypes and curated references for all genotypes (concatenated display).
Disease models
Find Mice (IMSR)
Mouse strains and cell lines available from the International Mouse Strain Resource (IMSR)
Carrying this Mutation:  Mouse Strains: 2 strains available      Cell Lines: 0 lines available
Carrying any Cd86 Mutation:  26 strains or lines available
Original:  J:39089 Borriello F, et al., B7-1 and B7-2 have overlapping, critical roles in immunoglobulin class switching and germinal center formation. Immunity. 1997 Mar;6(3):303-13
All:  109 reference(s)

Contributing Projects:
Mouse Genome Database (MGD), Gene Expression Database (GXD), Mouse Models of Human Cancer database (MMHCdb) (formerly Mouse Tumor Biology (MTB), Gene Ontology (GO)
Citing These Resources
Funding Information
Warranty Disclaimer & Copyright Notice
Send questions and comments to User Support.
last database update
MGI 6.14
The Jackson Laboratory