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Nos3tm1Gdk
Targeted Allele Detail
Nomenclature
Symbol: Nos3tm1Gdk
Name: nitric oxide synthase 3, endothelial cell; targeted mutation 1, Axel Godecke
MGI ID: MGI:2159003
Synonyms: eNOS-
Gene: Nos3  Location: Chr5:24364810-24384474 bp, + strand  Genetic Position: Chr5, 11.32 cM
Mutation
origin
Germline Transmission:  Earliest citation of germline transmission: J:46061
Parent Cell Line:  E14.1 (ES Cell)
Strain of Origin:  129P2/OlaHsd
Mutation
description
Allele Type:    Targeted (Null/knockout)
Mutations:    Insertion, Intragenic deletion
 
Mutation detailsA genomic fragment containing exons 24 and part of 25 was replaced with a neomycin selection cassette. The deleted sequences encode the NADPH binding sites. Western blot analysis on extracts of heart, lung, kidney and liver, and immunohistochemistry experiments on homozygous mice confirmed that no stable protein was expressed from this allele. (J:46061, J:62375)
Phenotypes
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View phenotypes for all genotypes (concatenated display).
Disease models
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Find Mice (IMSR)
Mouse strains and cell lines available from the International Mouse Strain Resource (IMSR)
Carrying this Mutation:  Mouse Strains: 0 strains available      Cell Lines: 0 lines available
Carrying any Nos3 Mutation:  7 strains or lines available
References
Original:  J:46061 Godecke A, et al., Coronary hemodynamics in endothelial NO synthase knockout mice. Circ Res. 1998 Feb 9;82(2):186-94
All:  23 reference(s)

Contributing Projects:
Mouse Genome Database (MGD), Gene Expression Database (GXD), Mouse Tumor Biology (MTB), Gene Ontology (GO), MouseCyc
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last database update
03/17/2015
MGI 5.21
The Jackson Laboratory