Camk2a^tm2Sva
Targeted Allele Detail

Summary

• Symbol: Camk2a^tm2Sva
• Name: calcium/calmodulin-dependent protein kinase II alpha; targeted mutation 2, Alcino Silva
• MGI ID: MGI:2158733
• Synonyms: alphaCaMK11^T286A, alphaCaMKII^{T286A-129B6F2}, Camk2a^T286A
• Gene: Camk2a Location: Chr18:61058704-61121224 bp, + strand Genetic Position: Chr18, 34.41 cM
• Alliance: Camk2a^tm2Sva page

Mutation origin

• Germline Transmission: Earliest citation of germline transmission: J:45750
• Parent Cell Line: R1 (ES Cell)
• Strain of Origin: (129X1/SvJ x 129S1/Sv)F1-Kitl⁺

Mutation description

• Allele Type: Targeted
• Mutations: Insertion, Nucleotide substitutions
• Mutation details: Point mutations were introduced into the gene that altered codon 286 from one encoding threonine to one encoding alanine. This mutation had been shown in vitro to eliminate autophosphorylation at this site and result in the elimination of calcium-calmodulin independent activity without affecting calcium-calmodulin dependent activity. An adjacent loxP-flanked neomycin cassette was removed by transient Cre mediated recombination in ES cells prior to the production of chimeric mice. Western blot and immunohistochemistry experiments on homozygous mice confirmed that a stable mutant protein was produced from this allele. (J:45750)

Find Mice (IMSR)

• Mouse strains and cell lines available from the International Mouse Strain Resource (IMSR)
• Carrying this Mutation: Mouse Strains: 1 strain available Cell Lines: 0 lines available
• Carrying any Camk2a Mutation: 136 strains or lines available

References

• Original: J:45750 Giese KP, et al., Autophosphorylation at Thr286 of the alpha calcium-calmodulin kinase II in LTP and learning. Science. 1998 Feb 6;279(5352):870-3
• All: 37 reference(s)