Chrnb4^tm1Mdb
Targeted Allele Detail

Summary

• Symbol: Chrnb4^tm1Mdb
• Name: cholinergic receptor, nicotinic, beta polypeptide 4; targeted mutation 1, Mariella De Biasi
• MGI ID: MGI:2158716
• Synonyms: beta4 -
• Gene: Chrnb4 Location: Chr9:54935438-54956063 bp, - strand Genetic Position: Chr9, 29.84 cM
• Alliance: Chrnb4^tm1Mdb page

Mutation origin

• Germline Transmission: Earliest citation of germline transmission: J:73590
• Parent Cell Line: AB2.2 (ES Cell)
• Strain of Origin: 129S7/SvEvBrd-Hprt1^b-m2

Mutation description

• Allele Type: Targeted (Null/knockout)
• Mutations: Insertion, Intragenic deletion
• Mutation details: A 4.1 kb genomic fragment containing exon 5 was replaced with a puromycin selection gene and an hprt minigene. RT-PCR analysis on RNA derived from brain of homozygous mice demonstrated that no detectable transcript is produced from this allele. (J:73590)

Expression

In Mice Carrying this Mutation:	1 RNA-Seq or microarray experiment(s)
In Structures Affected by this Mutation:	1 anatomical structure(s)

Find Mice (IMSR)

• Mouse strains and cell lines available from the International Mouse Strain Resource (IMSR)
• Carrying this Mutation: Mouse Strains: 2 strains available Cell Lines: 0 lines available
• Carrying any Chrnb4 Mutation: 22 strains or lines available

References

• Original: J:73590 Xu W, et al., Multiorgan autonomic dysfunction in mice lacking the beta2 and the beta4 subunits of neuronal nicotinic acetylcholine receptors. J Neurosci. 1999 Nov 1;19(21):9298-305
• All: 31 reference(s)