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Targeted Allele Detail
Symbol: Esr1tm1Ksk
Name: estrogen receptor 1 (alpha); targeted mutation 1, Kenneth S Korach
MGI ID: MGI:2150897
Synonyms: AERKO, alphaERKO, alpha-ERKO, ER1KO, ER-alpha-, ERalpha-, ERalphaKO, ERalpha-KO, ERalphaKOCH, ERa-Neo, ERKO, Esr1tm1Ksk, Esr1tm1Unc
Gene: Esr1  Location: Chr10:4611989-5005633 bp, + strand  Genetic Position: Chr10, 2.03 cM
Germline Transmission:  Earliest citation of germline transmission: J:16338
Parent Cell Line:  E14TG2a (ES Cell)
Strain of Origin:  129P2/OlaHsd
Allele Type:    Targeted (Null/knockout)
Mutation:    Insertion
Mutation detailsInsertion of a PGK-neomycin resistance cassette into exon 2 disrupted the reading frame downstream of the ATG start site. ELISA studies using antibodies to the carboxy terminus detected low concentrations of endogenous protein in uterine tissue from homozygous mutant mice. RT-PCR analysis detected two truncated mutant transcripts in uterine tissue from homozygous mice. Western blot analysis detected a 61 kDa protein in uterine tissue from homozygotes. Transient transfection assays confirmed that this protein retains some transactivation activity in the presence of estradiol. (J:16338, J:105821, J:112080)
View phenotypes and curated references for all genotypes (concatenated display).
In Mice Carrying this Mutation: 3 assay results
In Structures Affected by this Mutation: 43 anatomical structures
Find Mice (IMSR)
Mouse strains and cell lines available from the International Mouse Strain Resource (IMSR)
Carrying this Mutation:  Mouse Strains: 2 strains available      Cell Lines: 0 lines available
Carrying any Esr1 Mutation:  14 strains or lines available
Original:  J:16338 Lubahn DB, et al., Alteration of reproductive function but not prenatal sexual development after insertional disruption of the mouse estrogen receptor gene. Proc Natl Acad Sci U S A. 1993 Dec 1;90(23):11162-6
All:  262 reference(s)

Contributing Projects:
Mouse Genome Database (MGD), Gene Expression Database (GXD), Mouse Models of Human Cancer database (MMHCdb) (formerly Mouse Tumor Biology (MTB), Gene Ontology (GO)
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last database update
MGI 6.15
The Jackson Laboratory