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Targeted Allele Detail
Symbol: Nos3tm1Plh
Name: nitric oxide synthase 3, endothelial cell; targeted mutation 1, Paul L Huang
MGI ID: MGI:2150145
Synonyms: eNOS-, eNOS KO, NOSIII-
Gene: Nos3  Location: Chr5:24364810-24384474 bp, + strand  Genetic Position: Chr5, 11.32 cM
Renal tubular apoptosis, regeneration, glomerulosclerosis and glomerular thrombus formation in Nos1tm1Plh/Nos1tm1Plh Nos2tm1Mrl/Nos2tm1Mrl Nos3tm1Plh/Nos3tm1Plh mice

Show the 1 phenotype image(s) involving this allele.

Germline Transmission:  Earliest citation of germline transmission: J:28778
Parent Cell Line:  J1 (ES Cell)
Strain of Origin:  129S4/SvJae
Allele Type:    Targeted (Null/knockout)
Mutations:    Insertion, Intragenic deletion
Mutation detailsA neomycin cassette replaced 3 exons that encode the NADPH ribose and adenine binding sites of the protein (amino acids 1010 - 1144). Western blots of protein extracts of brain, heart, lung, and aorta/vena cava from homozygous mutant mice showed no detectable encoded protein. (J:28778)
Inheritance:    Dominant
View phenotypes for all genotypes (concatenated display).
Disease models
Find Mice (IMSR)
Mouse strains and cell lines available from the International Mouse Strain Resource (IMSR)
Carrying this Mutation:  Mouse Strains: 1 strain available      Cell Lines: 0 lines available
Carrying any Nos3 Mutation:  7 strains or lines available
Mice homozygous for Nos1tm1Plh and Nos3tm1Plh display Phenotypic Similarity to Human Syndrome: hypertensive hypertrophic cardiomyopathy of the elderly (J:129064)

Original:  J:28778 Huang PL, et al., Hypertension in mice lacking the gene for endothelial nitric oxide synthase [see comments]. Nature. 1995 Sep 21;377(6546):239-42
All:  119 reference(s)

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Mouse Genome Database (MGD), Gene Expression Database (GXD), Mouse Tumor Biology (MTB), Gene Ontology (GO), MouseCyc
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last database update
MGI 6.01
The Jackson Laboratory