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Targeted Allele Detail
Symbol: Ppargtm1Rev
Name: peroxisome proliferator activated receptor gamma; targeted mutation 1, Ronald M Evans
MGI ID: MGI:2149838
Synonyms: Pparg-, PPARg-
Gene: Pparg  Location: Chr6:115361221-115490401 bp, + strand  Genetic Position: Chr6, 53.41 cM, cytoband E3-F1
Germline Transmission:  Earliest citation of germline transmission: J:58223
Parent Cell Line:  J1 (ES Cell)
Strain of Origin:  129S4/SvJae
Allele Type:    Targeted (Null/knockout, Reporter)
Mutation:    Insertion
Mutation detailsIn-frame insertion of a lac Z-neomycin resistance cassette into the second coding exon of the gene, just upstream of the DNA binding domain, eliminated both DNA-binding and ligand-binding functions of the receptor. Whole-mount beta-galactosidase assays revealed conspicuous beta-gal activivity in the interscapular brown fat pad of only E14.5 embryos carrying a targeted allele, but not in wild-type embryos (J:58223)
View phenotypes for all genotypes (concatenated display).
Disease models
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Mouse strains and cell lines available from the International Mouse Strain Resource (IMSR)
Carrying this Mutation:  Mouse Strains: 1 strain available      Cell Lines: 0 lines available
Carrying any Pparg Mutation:  16 strains or lines available
Complementation of Ppargtm1Rev mutant embryos with wild-type placenta by tetraploid aggregation corrects the cardiac defect and extends viability to E12.5 (two mutants out of a total five), E15.5 (one out of ten) and at birth (one of six). Rescued embryos survive to term but exhibit a second lethal phase during the first week of life. Additional phenotypes include: a 30% reduction in body weight (P0-P4) and subsequent ill health (as shown by dehydration, weight loss and lethargy starting at P5) associated with lipodystrophy, a severly pale, distended and fatty liver, severe intestinal bleeding, and numerous focal hematomas throughout the brain (J:58223).

Original:  J:58223 Barak Y, et al., PPAR gamma is required for placental, cardiac, and adipose tissue development. Mol Cell. 1999 Oct;4(4):585-95
All:  13 reference(s)

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