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Targeted Allele Detail
Symbol: Apoetm1Bres
Name: apolipoprotein E; targeted mutation 1, Jan L Breslow
MGI ID: MGI:2137814
Synonyms: apo E-, apoE-, EKO, epsilon-
Gene: Apoe  Location: Chr7:19430034-19433113 bp, - strand  Genetic Position: Chr7, 9.94 cM
Gross appearance of aortic arches of Apoetm1Bres/Apoetm1Bres, Plgtm1Jld/Plgtm1Jld and Apoetm1Bres/Apoetm1Bres Plgtm1Jld/Plgtm1Jld mice

Show the 3 phenotype image(s) involving this allele.

Germline Transmission:  Earliest citation of germline transmission: J:2809
Parent Cell Line:  E14 (ES Cell)
Strain of Origin:  129P2/OlaHsd
Allele Type:    Targeted (Null/knockout)
Mutations:    Insertion, Intragenic deletion
Mutation detailsA neomycin resistance cassette replaced 1.4kb of the gene, including part of the promoter through part of the second intron. Northern blot analysis using a mouse exon 4 riboprobe did not detect wild-type message in livers from homozygous mutant mice, but did detect a longer transcript that the authors propose is a neo-Apoe fusion product. Gradient SDS-PAGE analysis did not detect full size Apoe associated with any fractions from plasma lipoproteins from homozygous mutant mice. (J:2809)
View phenotypes and curated references for all genotypes (concatenated display).
In Structures Affected by this Mutation: 4 anatomical structures
Find Mice (IMSR)
Mouse strains and cell lines available from the International Mouse Strain Resource (IMSR)
Carrying this Mutation:  Mouse Strains: 12 strains available      Cell Lines: 0 lines available
Carrying any Apoe Mutation:  132 strains or lines available

In Apoetm1Bres mutant mice also carrying Csf1op, atherotic lesions of the proximal aorta were abolished or significantly reduced in size relative to Apoe mutants homozygous for the normal allele at Csf1 (J:29024).

Original:  J:2809 Plump AS, et al., Severe hypercholesterolemia and atherosclerosis in apolipoprotein E-deficient mice created by homologous recombination in ES cells. Cell. 1992 Oct 16;71(2):343-53
All:  133 reference(s)

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Mouse Genome Database (MGD), Gene Expression Database (GXD), Mouse Models of Human Cancer database (MMHCdb) (formerly Mouse Tumor Biology (MTB), Gene Ontology (GO)
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MGI 6.17
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