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Targeted Allele Detail
Symbol: Krastm2Tyj
Name: Kirsten rat sarcoma viral oncogene homolog; targeted mutation 2, Tyler Jacks
MGI ID: MGI:2135928
Synonyms: K-ras2LA1, K-rasLA1, KrasLA1
Gene: Kras  Location: Chr6:145216699-145250239 bp, - strand  Genetic Position: Chr6, 77.37 cM, cytoband G2
Germline Transmission:  Earliest citation of germline transmission: J:68981
Parent Cell Line:  D3 (ES Cell)
Strain of Origin:  129S2/SvPas
Allele Type:    Targeted
Mutations:    Insertion, Nucleotide substitutions
Mutation detailsInsertion of the targeting vector results in a duplication of exon 1 with a neomycin resistance gene between the duplicate exons. The upstream (inserted) exon 1 contains an activating glycine to aspartic acid mutation at codon 12. In the absence of intrachromosomal recombination, this allele does not produce any protein and mutant homozygotes die during embryogenesis. In heterozygotes, when intrachromosomal recombination occurs in vivo, half of the recombination events produce an active mutant allele coding for the G12D amino acid substitution and half produce a wild-type allele. Expression of G12D protein results in the formation of lung tumors and death at ~300 days (10 months). (J:68981)
View phenotypes and curated references for all genotypes (concatenated display).
Disease models
In Structures Affected by this Mutation: 5 anatomical structures
Find Mice (IMSR)
Mouse strains and cell lines available from the International Mouse Strain Resource (IMSR)
Carrying this Mutation:  Mouse Strains: 1 strain available      Cell Lines: 0 lines available
Carrying any Kras Mutation:  30 strains or lines available
Original:  J:68981 Johnson L, et al., Somatic activation of the K-ras oncogene causes early onset lung cancer in mice. Nature. 2001 Apr 26;410(6832):1111-6
All:  29 reference(s)

Contributing Projects:
Mouse Genome Database (MGD), Gene Expression Database (GXD), Mouse Tumor Biology (MTB), Gene Ontology (GO), MouseCyc
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MGI 6.13
The Jackson Laboratory