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Selltm1Hyn
Targeted Allele Detail
Nomenclature
Symbol: Selltm1Hyn
Name: selectin, lymphocyte; targeted mutation 1, Richard Hynes
MGI ID: MGI:1934022
Synonyms: L-, L-Sel-
Gene: Sell  Location: Chr1:164061982-164084181 bp, + strand  Genetic Position: Chr1, 71.37 cM
Mutation
origin
Germline Transmission:  Earliest citation of germline transmission: J:57973
Parent Cell Line:  D3 (ES Cell)
Strain of Origin:  129S2/SvPas
Mutation
description
Allele Type:    Targeted (Null/knockout)
Mutations:    Insertion, Intragenic deletion
 
Mutation detailsThe three selectin genes, Sele, Sell, and Selp, are found within a 300 kb genomic region. In order to create a triple knockout, ES cells heterozygote for the Selptm1Hyn and Seletm1Hyn null alleles in cis to each other were targeted a third time. A PGK-puro cassette was used to delete a 2.1kb region containing the exons encoding the lectin domain and most of the epidermal growth factor domain. Flow cytometric analysis showed an absence of all three encoded proteins in leukocytes isolated from homozygotes. This targeted mutation occurred in cis with the other two null mutations. Due to their genomic proximity, the three null alleles should propagate together through the offspring. (J:57973)
Phenotypes
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Find Mice (IMSR)
Mouse strains and cell lines available from the International Mouse Strain Resource (IMSR)
Carrying this Mutation:  Mouse Strains: 5 strains available      Cell Lines: 0 lines available
Carrying any Sell Mutation:  23 strains or lines available
References
Original:  J:57973 Robinson SD, et al., Multiple, targeted deficiencies in selectins reveal a predominant role for P-selectin in leukocyte recruitment. Proc Natl Acad Sci U S A. 1999 Sep 28;96(20):11452-7
All:  22 reference(s)

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Mouse Genome Database (MGD), Gene Expression Database (GXD), Mouse Tumor Biology (MTB), Gene Ontology (GO), MouseCyc
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last database update
07/19/2016
MGI 6.04
The Jackson Laboratory