Cnr1^tm1Zim
Targeted Allele Detail

Summary

• Symbol: Cnr1^tm1Zim
• Name: cannabinoid receptor 1; targeted mutation 1, Andreas Zimmer
• MGI ID: MGI:1857762
• Synonyms: Cb1^-, CB1^neor, CB1R KO, Cnr1-
• Gene: Cnr1 Location: Chr4:33924593-33948831 bp, + strand Genetic Position: Chr4, 16.28 cM
• Alliance: Cnr1^tm1Zim page

Mutation origin

• Germline Transmission: Earliest citation of germline transmission: J:54995
• Parent Cell Line: MPI-II (ES Cell)
• Strain of Origin: 129S2/SvPas

Mutation description

• Allele Type: Targeted (Null/knockout)
• Mutations: Insertion, Intragenic deletion
• Mutation details: This Cnr1 mutation was created by gene targeting in which a neo cassette replaced amino acids 33-447 of the coding region (J:54995). A neomycin resistance cassette replaced genomic sequence coding for amino acids 33-447, corresponding to most of the coding sequence of the gene. Absence of the encoded protein in homozygous animals was confirmed by receptor binding studies. (J:54995)

Expression

In Mice Carrying this Mutation:	1 assay results 1 RNA-Seq or microarray experiment(s)
In Structures Affected by this Mutation:	1 anatomical structure(s)

Find Mice (IMSR)

• Mouse strains and cell lines available from the International Mouse Strain Resource (IMSR)
• Carrying this Mutation: Mouse Strains: 2 strains available Cell Lines: 0 lines available
• Carrying any Cnr1 Mutation: 43 strains or lines available

References

• Original: J:54995 Zimmer A, et al., Increased mortality, hypoactivity, and hypoalgesia in cannabinoid CB1 receptor knockout mice [see comments]. Proc Natl Acad Sci U S A. 1999 May 11;96(10):5780-5
• All: 147 reference(s)