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Pemttm1J
Targeted Allele Detail
Nomenclature
Symbol: Pemttm1J
Name: phosphatidylethanolamine N-methyltransferase; targeted mutation 1, Jackson
MGI ID: MGI:1857394
Synonyms: Pempt2-, Pemt-
Gene: Pemt  Location: Chr11:59861440-59937315 bp, - strand  Genetic Position: Chr11, 37.81 cM
Small size of Pemttm1J/Pemttm1J tnyw/tnyw mice (left) with littermate control (right) at 2 weeks of age

Show the 3 phenotype image(s) involving this allele.

Mutation
origin
Germline Transmission:  Earliest citation of germline transmission: J:44607
Parent Cell Line:  E14 (ES Cell)
Strain of Origin:  129P2/OlaHsd
Mutation
description
Allele Type:    Targeted (Null/knockout)
Mutations:    Insertion, Intragenic deletion
 
Mutation detailsA PGK neomycin cassette replaces the majority of exon 2, including the translation start site and the extreme 5' end of the intron. Lack of protein in homozygous mutant mice was demonstrated by immunoblot assays on liver homogenates, using a rat antibody from the C terminal end of the protein. Enzymatic activity was also abolished in homozygous mutant animals. (J:44607)
Phenotypes
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View phenotypes and curated references for all genotypes (concatenated display).
Expression
In Structures Affected by this Mutation: 2 anatomical structures
Find Mice (IMSR)
Mouse strains and cell lines available from the International Mouse Strain Resource (IMSR)
Carrying this Mutation:  Mouse Strains: 2 strains available      Cell Lines: 0 lines available
Carrying any Pemt Mutation:  10 strains or lines available
References
Original:  J:44607 Walkey CJ, et al., Disruption of the murine gene encoding phosphatidylethanolamine N-methyltransferase. Proc Natl Acad Sci U S A. 1997 Nov 25;94(24):12880-5
All:  35 reference(s)

Contributing Projects:
Mouse Genome Database (MGD), Gene Expression Database (GXD), Mouse Models of Human Cancer database (MMHCdb) (formerly Mouse Tumor Biology (MTB), Gene Ontology (GO)
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last database update
11/30/2021
MGI 6.17
The Jackson Laboratory