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Targeted Allele Detail
Symbol: Prf1tm1Sdz
Name: perforin 1 (pore forming protein); targeted mutation 1, Sandoz Pharmaceuticals
MGI ID: MGI:1857235
Synonyms: P0, perf-, perforin-, perforin 0, Pfn-, pfp-, pfpKO, Pfptm1Sdz, pko, Prf-, Prf1-, prf1tm1, Prf1tm/Sdz
Gene: Prf1  Location: Chr10:61297833-61304680 bp, + strand  Genetic Position: Chr10, 32.18 cM
Germline Transmission:  Earliest citation of germline transmission: J:17986
Parent Cell Line:  BL/6-III (ES Cell)
Strain of Origin:  C57BL/6J
Allele Type:    Targeted (Null/knockout)
Mutation:    Insertion
Mutation detailsA neomycin selection cassette was inserted into exon 3. RT-PCR analysis on RNA derived from homozygous mice demonstrated that an abnormal transcript was produced from this allele. However, immunocytochemistry experiments on activated spleen cells derived from homozygous mice confirmed that no detectable protein was made from this allele. (J:17986, J:96542)
View phenotypes and curated references for all genotypes (concatenated display).
Disease models
In Structures Affected by this Mutation: 13 anatomical structures
Find Mice (IMSR)
Mouse strains and cell lines available from the International Mouse Strain Resource (IMSR)
Carrying this Mutation:  Mouse Strains: 12 strains available      Cell Lines: 0 lines available
Carrying any Prf1 Mutation:  38 strains or lines available
Original:  J:17986 Kagi D, et al., Cytotoxicity mediated by T cells and natural killer cells is greatly impaired in perforin-deficient mice [see comments]. Nature. 1994 May 5;369(6475):31-7
All:  315 reference(s)

Contributing Projects:
Mouse Genome Database (MGD), Gene Expression Database (GXD), Mouse Models of Human Cancer database (MMHCdb) (formerly Mouse Tumor Biology (MTB), Gene Ontology (GO)
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last database update
MGI 6.16
The Jackson Laboratory