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Ngfrtm1Jae
Targeted Allele Detail
Nomenclature
Symbol: Ngfrtm1Jae
Name: nerve growth factor receptor (TNFR superfamily, member 16); targeted mutation 1, Rudolf Jaenisch
MGI ID: MGI:1857226
Synonyms: Ngfr-, NGFRtm1Jac, p75-, p75e3-, p75exonIII-, p75(III)-, p75-KO, p75NGFR, p75NTR-, p75NTR-, p75NTRexon3-null, p75NTR/ExonIII-, p75NTR KO
Gene: Ngfr  Location: Chr11:95568818-95587735 bp, - strand  Genetic Position: Chr11, 59.01 cM
Reduced infarct size in Ngfrtm1Jae/Ngfrtm1Jae mice after cardiac ischemia-reperfusion surgery

Show the 3 phenotype image(s) involving this allele.

Mutation
origin
Germline Transmission:  Earliest citation of germline transmission: J:43748
Parent Cell Line:  J1 (ES Cell)
Strain of Origin:  129S4/SvJae
Mutation
description
Allele Type:    Targeted (Null/knockout)
Mutation:    Insertion
 
Mutation detailsA neomycin selection cassette was inserted into the third exon of the gene, disrupting the sequences encoding cysteine repeats 2, 3, and 4. Northern blot analysis revealed that the mutant gene did not yield a full length mRNA, however subsequent RT-PCR analysis, described in J:71955, detected an endogenous alternative transcript which lacks exon 3. Western blot analysis showed that the full length isoform was absent in homozygous mutant mice, but an isoform lacking cysteine repeats 2, 3, and 4 was present in both wild and mutant mice. In vitro experiments showed the persisting isoform to be a transmembrane protein that cannot bind neurotrophins but interacts with tyrosine kinase receptors. (J:43748, J:71955)
Phenotypes
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Expression
In Mice Carrying this Mutation: 21 assay results
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Mouse strains and cell lines available from the International Mouse Strain Resource (IMSR)
Carrying this Mutation:  Mouse Strains: 2 strains available      Cell Lines: 0 lines available
Carrying any Ngfr Mutation:  13 strains or lines available
References
Original:  J:43748 Lee KF, et al., Targeted mutation of the gene encoding the low affinity NGF receptor p75 leads to deficits in the peripheral sensory nervous system. Cell. 1992 May 29;69(5):737-49
All:  171 reference(s)

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Mouse Genome Database (MGD), Gene Expression Database (GXD), Mouse Tumor Biology (MTB), Gene Ontology (GO), MouseCyc
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last database update
08/23/2016
MGI 6.05
The Jackson Laboratory