Cd28^tm1Mak
Targeted Allele Detail

Summary

• Symbol: Cd28^tm1Mak
• Name: CD28 antigen; targeted mutation 1, Tak Mak
• MGI ID: MGI:1857150
• Synonyms: CD28^-, CD28KO
• Gene: Cd28 Location: Chr1:60785547-60812521 bp, + strand Genetic Position: Chr1, 30.52 cM, cytoband C1-C3
• Alliance: Cd28^tm1Mak page

Mutation origin

• Germline Transmission: Earliest citation of germline transmission: J:14194
• Parent Cell Line: D3 (ES Cell)
• Strain of Origin: 129S2/SvPas

Mutation description

• Allele Type: Targeted (Null/knockout)
• Mutations: Insertion, Intragenic deletion
• Mutation details: A genomic fragment containing part of exon 2 was replaced by a neomycin resistance cassette. Flow-cytometry analysis on peripheral blood lymphocytes demonstrated that the protein was not expressed in these cells in homozygous mice. (J:14194)

Expression

In Structures Affected by this Mutation:

4 anatomical structure(s)

Find Mice (IMSR)

• Mouse strains and cell lines available from the International Mouse Strain Resource (IMSR)
• Carrying this Mutation: Mouse Strains: 12 strains available Cell Lines: 0 lines available
• Carrying any Cd28 Mutation: 53 strains or lines available

References

• Original: J:14194 Shahinian A, et al., Differential T cell costimulatory requirements in CD28-deficient mice. Science. 1993 Jul 30;261(5121):609-12
• All: 312 reference(s)