Summary | Mutation origin | Mutation description | Expression | Phenotypes | Find Mice (IMSR) | References

Symbol: Slirptm1.2Pjle Name: SRA stem-loop interacting RNA binding protein; targeted mutation 1.2, Peter J Leedman MGI ID: MGI:5538716 Gene: Slirp  Location: Chr12:87490666-87496694 bp, + strand  Genetic Position: Chr12, 41.67 cM, cytoband E Alliance: Slirptm1.2Pjle page

Annulus and mitochondrial disruption in Slirptm1.2Pjle/Slirptm1.2Pjle sperm Show the 2 phenotype image(s) involving this allele.

Germline Transmission:	Earliest citation of germline transmission: J:204433
Parent Cell Line:	Not Specified (ES Cell)
Strain of Origin:	Not Specified

Allele Type:    Targeted (Null/knockout) Mutations:    Insertion, Intragenic deletion   Mutation details: Exon 2 was replaced with loxP-flanked cDNA for exons 2 to 4, with splice acceptor site at the 5' end and a polyA signal at the 3' end. Cre recombinase removed the loxP-flanked sequence, leaving behind a loxP site and downstream artificial exon with a splice acceptor, Flag epitope coding sequence, stop codon and c-fos sequence. Western blot analysis confirmed the absence of wild-type protein in testis lysates from homozygous mice. (J:204433)

Generation of the Slirptm1.2Pjle allele

View phenotypes and curated references for all genotypes (concatenated display).

In Structures Affected by this Mutation:

1 anatomical structure(s)

Mouse strains and cell lines available from the International Mouse Strain Resource (IMSR)
Carrying this Mutation:	Mouse Strains: 0 strains available      Cell Lines: 0 lines available
Carrying any Slirp Mutation:	20 strains or lines available

Original:	J:204433 Colley SM, et al., Loss of the nuclear receptor corepressor SLIRP compromises male fertility. PLoS One. 2013;8(8):e70700
All:	1 reference(s)