Summary | Mutation origin | Mutation description | Phenotypes | Find Mice (IMSR) | References

Symbol:	Itgb3tm1Aja
Name:	integrin beta 3; targeted mutation 1, Ararat J Ablooglu
MGI ID:	MGI:3844533
Synonyms:	Beta3(delta760-762)
Gene:	Itgb3  Location: Chr11:104498826-104561302 bp, + strand  Genetic Position: Chr11, 67.84 cM
Alliance:	Itgb3tm1Aja page

Germline Transmission:	Earliest citation of germline transmission: J:148306
Parent Cell Line:	R1 (ES Cell)
Strain of Origin:	(129X1/SvJ x 129S1/Sv)F1-Kitl+

Allele Type:    Targeted (Null/knockout) Mutations:    Insertion, Intragenic deletion   Mutation details: The sequence encoding the final three C-terminal residues (arginine-glycine-threonine) that are necessary for interaction with Src kinase were targeted for removal. Targeting constructs also contained several silent mutations to introduce new HpaI and NdeI restriction sites and a floxed neomycin-resistance cassette between exons 14 and 15. Mice carrying the mutation were crossed with cre transgenic mice to remove the neomycin selection cassette. Extracellular protein expression levels on homozygous platelets was only 59% of wild-type levels, which was similar to that found on heterozygous null platelets. (J:148306)

View phenotypes and curated references for all genotypes (concatenated display).

Mouse strains and cell lines available from the International Mouse Strain Resource (IMSR)
Carrying this Mutation:	Mouse Strains: 0 strains available      Cell Lines: 0 lines available
Carrying any Itgb3 Mutation:	43 strains or lines available

Original:	J:148306 Ablooglu AJ, et al., Antithrombotic effects of targeting alphaIIbbeta3 signaling in platelets. Blood. 2009 Apr 9;113(15):3585-92
All:	3 reference(s)