Summary | Mutation origin | Mutation description | Expression | Phenotypes | Find Mice (IMSR) | References

Symbol:	Rspo1tm1.1Ktom
Name:	R-spondin 1; targeted mutation 1.1, Kazuma Tomizuka
MGI ID:	MGI:3784965
Gene:	Rspo1  Location: Chr4:124880223-124902892 bp, + strand  Genetic Position: Chr4, 57.96 cM
Alliance:	Rspo1tm1.1Ktom page

Germline Transmission:	Earliest citation of germline transmission: J:133934
Parent Cell Line:	TT2 (ES Cell)
Strain of Origin:	(C57BL/6NCrlj x CBA/JNCrlj)F1

Allele Type:    Targeted (Null/knockout) Mutations:    Insertion, Intragenic deletion   Mutation details: This allele was made using sequential targeting events. The first targeting event resulted in the insertion of a floxed neo cassette into exon 2 and the second event resulted in the replacement of exons 5 through 6 with a floxed puromycin resistance cassette. Transient cre expression was used to remove the selection cassettes and the remaining coding region of exon 2 and exon 3. The final allele lacks the entire coding region. The absence of transcript was confirmed by RT-PCR on ovary and testis extracts. (J:133934)

View phenotypes and curated references for all genotypes (concatenated display).

In Structures Affected by this Mutation:

3 anatomical structure(s)

Mouse strains and cell lines available from the International Mouse Strain Resource (IMSR)
Carrying this Mutation:	Mouse Strains: 0 strains available      Cell Lines: 0 lines available
Carrying any Rspo1 Mutation:	21 strains or lines available

Original:	J:133934 Tomizuka K, et al., R-spondin1 plays an essential role in ovarian development through positively regulating Wnt-4 signaling. Hum Mol Genet. 2008 May 1;17(9):1278-91
All:	1 reference(s)