Summary | Mutation origin | Mutation description | Expression | Phenotypes | Find Mice (IMSR) | References

Symbol:	Trpa1tm1Kykw
Name:	transient receptor potential cation channel, subfamily A, member 1; targeted mutation 1, Kelvin Y Kwan
MGI ID:	MGI:3624810
Synonyms:	Trpa1 KO
Gene:	Trpa1  Location: Chr1:14942872-14989086 bp, - strand  Genetic Position: Chr1, 4.5 cM
Alliance:	Trpa1tm1Kykw page

Germline Transmission:	Earliest citation of germline transmission: J:108343
Parent Cell Line:	E14 (ES Cell)
Strain of Origin:	129P2/OlaHsd

Allele Type:    Targeted (Null/knockout) Mutations:    Insertion, Intragenic deletion   Mutation details: The S5 and S6 transmembrane domains and the pore loop containing the selectivity filter of the gene were replaced with an IRES-PLAP-pA cassette. The resulting sequence produces a bicistronic transcript containing the 5' end of the endogenous gene followed by an independently translated human placental alkaline phosphatase gene. An endoplasmic reticulum retention signal encoded by the amino acid sequence KDEL and a stop codon were inserted in frame with the exon before the IRES-PLAP to prevent unwanted side effects from a possibly truncated product of the endogenous coding sequence. RT-PCR confirmed absence of transcript in mutants. (J:108343)

View phenotypes and curated references for all genotypes (concatenated display).

In Structures Affected by this Mutation:

1 anatomical structure(s)

Mouse strains and cell lines available from the International Mouse Strain Resource (IMSR)
Carrying this Mutation:	Mouse Strains: 1 strain available      Cell Lines: 0 lines available
Carrying any Trpa1 Mutation:	92 strains or lines available

Original:	J:108343 Kwan KY, et al., TRPA1 contributes to cold, mechanical, and chemical nociception but is not essential for hair-cell transduction. Neuron. 2006 Apr 20;50(2):277-89
All:	139 reference(s)