About   Help   FAQ
Ndst1tm1.1Grob
Targeted Allele Detail
Summary
Symbol: Ndst1tm1.1Grob
Name: N-deacetylase/N-sulfotransferase (heparan glucosaminyl) 1; targeted mutation 1, Kay Grobe
MGI ID: MGI:3589383
Gene: Ndst1  Location: Chr18:60817566-60881722 bp, - strand  Genetic Position: Chr18, 34.38 cM, cytoband D2
Alliance: Ndst1tm1.1Grob page
Mutation
origin
Germline Transmission:  Earliest citation of germline transmission: J:100425
Parent Cell Line:  R1 (ES Cell)
Strain of Origin:  (129X1/SvJ x 129S1/Sv)F1-Kitl+
Mutation
description
Allele Type:    Targeted (Null/knockout)
Mutation:    Intragenic deletion
 
Mutation detailsCre mediated excision was used to remove exon 2, containing most of the 5' untranslated region and 171 of 882 amino acids of the open reading frame, containing the signal peptide, cytoplasmic tail, transmembrane region and part of the catalytic domain. Western blot of mutant embryo extract demonstrated lack of protein. In situ hybridization of E11.5 embryos failed to detect reactivity using an antisense riboprobe. Immunohistochemistry also confirmed successful protein ablation. (J:100425)
Phenotypes
Loading...
View phenotypes and curated references for all genotypes (concatenated display).
Expression
In Structures Affected by this Mutation: 20 anatomical structure(s)
Find Mice (IMSR)
Mouse strains and cell lines available from the International Mouse Strain Resource (IMSR)
Carrying this Mutation:  Mouse Strains: 0 strains available      Cell Lines: 0 lines available
Carrying any Ndst1 Mutation:  46 strains or lines available
References
Original:  J:100425 Grobe K, et al., Cerebral hypoplasia and craniofacial defects in mice lacking heparan sulfate Ndst1 gene function. Development. 2005 Aug;132(16):3777-86
All:  6 reference(s)

Contributing Projects:
Mouse Genome Database (MGD), Gene Expression Database (GXD), Mouse Models of Human Cancer database (MMHCdb) (formerly Mouse Tumor Biology (MTB)), Gene Ontology (GO)
Citing These Resources
Funding Information
Warranty Disclaimer, Privacy Notice, Licensing, & Copyright
Send questions and comments to User Support.
last database update
03/12/2024
MGI 6.23
The Jackson Laboratory