Summary | Mutation origin | Mutation description | Expression | Phenotypes | Find Mice (IMSR) | References

Symbol:	Sema5atm1Awp
Name:	sema domain, seven thrombospondin repeats (type 1 and type 1-like), transmembrane domain (TM) and short cytoplasmic domain, (semaphorin) 5A; targeted mutation 1, Andreas W Puschel
MGI ID:	MGI:3577050
Synonyms:	Sema5a-
Gene:	Sema5a  Location: Chr15:32244959-32696487 bp, + strand  Genetic Position: Chr15, 13.02 cM
Alliance:	Sema5atm1Awp page

Germline Transmission:	Earliest citation of germline transmission: J:97663
Parent Cell Line:	R1 (ES Cell)
Strain of Origin:	(129X1/SvJ x 129S1/Sv)F1-Kitl+

Allele Type:    Targeted (Null/knockout) Mutations:    Insertion, Intragenic deletion   Mutation details: A targeting vector was designed to replace exons 4 and 5 with a PGK-neo cassette. The null allele was achieved by inserting a stop codon after amino acid 121, thereby rendering a truncated protein unlikely to be biological active. Alternatively, aberrant splicing between exons 3 and 6 would result in the generation of a stop codon after amino acid 141. Western blot analysis of membrane fractions demonstrated a complete lack of protein in mutants. (J:97663)

View phenotypes and curated references for all genotypes (concatenated display).

In Structures Affected by this Mutation:

2 anatomical structure(s)

Mouse strains and cell lines available from the International Mouse Strain Resource (IMSR)
Carrying this Mutation:	Mouse Strains: 0 strains available      Cell Lines: 0 lines available
Carrying any Sema5a Mutation:	65 strains or lines available

Original:	J:97663 Fiore R, et al., Inactivation of the Sema5a gene results in embryonic lethality and defective remodeling of the cranial vascular system. Mol Cell Biol. 2005 Mar;25(6):2310-9
All:	1 reference(s)