Summary | Mutation origin | Mutation description | Phenotypes | Find Mice (IMSR) | References

Symbol:	Hspg2tm1Soin
Name:	perlecan (heparan sulfate proteoglycan 2); targeted mutation 1, Raija Soininen
MGI ID:	MGI:2449915
Synonyms:	Hspg2delta3
Gene:	Hspg2  Location: Chr4:137196080-137297941 bp, + strand  Genetic Position: Chr4, 69.93 cM
Alliance:	Hspg2tm1Soin page

Germline Transmission:	Earliest citation of germline transmission: J:81399
Parent Cell Line:	E14 (ES Cell)
Strain of Origin:	129P2/OlaHsd

Allele Type:    Targeted (Null/knockout) Mutations:    Insertion, Intragenic deletion   Mutation details: Exon 3 and flanking regions were deleted and replaced with a PGK-neo cassette via homologous recombination. The targeted mutation removes the heparan sulfate attachment while the core protein remains intact. RT-PC analysis of RNA from homozygous mutant embryos confirmed the exon 3 deletion. Fibroblasts derived from homozygous mutant cell culture were treated with heparitinase and chondroitinase, run on an SDS-PAGE gel, and immunoblotted to confirm the mutant protein retained the core protein and lacked heparan sulfate side chains. (J:81399)

View phenotypes and curated references for all genotypes (concatenated display).

Mouse strains and cell lines available from the International Mouse Strain Resource (IMSR)
Carrying this Mutation:	Mouse Strains: 1 strain available      Cell Lines: 0 lines available
Carrying any Hspg2 Mutation:	308 strains or lines available

Original:	J:81399 Rossi M, et al., Heparan sulfate chains of perlecan are indispensable in the lens capsule but not in the kidney. EMBO J. 2003 Jan 15;22(2):236-45
All:	13 reference(s)