Summary | Mutation origin | Mutation description | Phenotypes | Find Mice (IMSR) | References

Symbol:	Mcf2tm1Ehi
Name:	mcf.2 transforming sequence; targeted mutation 1, Emilio Hirsch
MGI ID:	MGI:2388053
Gene:	Mcf2  Location: ChrX:59101316-59224449 bp, - strand  Genetic Position: ChrX, 33.5 cM, cytoband A6/A7
Alliance:	Mcf2tm1Ehi page

Germline Transmission:	Earliest citation of germline transmission: J:78933
Parent Cell Line:	R1 (ES Cell)
Strain of Origin:	(129X1/SvJ x 129S1/Sv)F1-Kitl+

Allele Type:    Targeted (Null/knockout) Mutation:    Insertion   Mutation details: A cassette containing IRES-lacZ followed by neo was inserted in exon 1 after the start codon via homologous recombination. Transcript was undetected by RT-PCR analysis of testis and brain tissue, while Western blot analysis confirmed the absence of protein in testis, brain, and liver tissue. LacZ expression was undetected by Xgal staining, putatively due to a combination of the low activity of the endogenous promoter and the inefficiency of the IRES sequence. (J:78933)

View phenotypes and curated references for all genotypes (concatenated display).

Mouse strains and cell lines available from the International Mouse Strain Resource (IMSR)
Carrying this Mutation:	Mouse Strains: 0 strains available      Cell Lines: 0 lines available
Carrying any Mcf2 Mutation:	14 strains or lines available

Original:	J:78933 Hirsch E, et al., Defective dendrite elongation but normal fertility in mice lacking the Rho-like GTPase activator Dbl. Mol Cell Biol. 2002 May;22(9):3140-8
All:	1 reference(s)