Elk3tm1Wlyk
Targeted Allele Detail
|
|
| Symbol: |
Elk3tm1Wlyk |
| Name: |
ELK3, member of ETS oncogene family; targeted mutation 1, Bohdan Wasylyk |
| MGI ID: |
MGI:2182950 |
| Synonyms: |
netdelta, Netmutant |
| Gene: |
Elk3 Location: Chr10:93083276-93146997 bp, - strand Genetic Position: Chr10, 48.04 cM, cytoband C-D1
|
| Alliance: |
Elk3tm1Wlyk page
|
|
| Germline Transmission: |
Earliest citation of germline transmission:
J:71816
|
| Parent Cell Line: |
D4 (ES Cell)
|
| Strain of Origin: |
129/Sv
|
|
| Allele Type: |
|
Targeted (Null/knockout) |
| Mutations: |
|
Insertion, Intragenic deletion
|
| |
|
Mutation details: Exon 2, which encodes the first 69 amino acids (including the initiator methionine), and surrounding intronic sequence were replaced with a neomycin selection cassette. RT-PCR and sequence analysis showed the presence of an aberrantly spliced transcript in which exon 1 had joined exon 3. The aberrant transcript was shown by Western blot analysis to produce a 37kDa protein via an in-frame translational start site at nucleotide 571 (aa 93). The mutant protein lacks the Ets DNA-binding domain.
(J:71816)
|
|
|
View phenotypes and curated references for all genotypes (concatenated display).
|
|
|
|
|
| Mouse strains and cell lines
available from the International Mouse Strain Resource
(IMSR) |
| Carrying this Mutation: |
Mouse Strains: 0 strains available
Cell Lines: 0 lines available
|
| Carrying any Elk3 Mutation: |
19 strains or lines available
|
|
| Original: |
J:71816 Ayadi A, et al., Net-targeted mutant mice develop a vascular phenotype and up-regulate egr-1. EMBO J. 2001 Sep 17;20(18):5139-52 |
| All: |
6 reference(s) |
|
Analysis Tools
