Summary | Mutation origin | Mutation description | Phenotypes | Find Mice (IMSR) | References

Symbol:	Lig1tm2Dwm
Name:	ligase I, DNA, ATP-dependent; targeted mutation 2, David W Melton
MGI ID:	MGI:2179196
Synonyms:	Lig1#12, Lig1-(no.12)
Gene:	Lig1  Location: Chr7:13011239-13045350 bp, + strand  Genetic Position: Chr7, 7.82 cM
Alliance:	Lig1tm2Dwm page

Germline Transmission:	Earliest citation of germline transmission: J:75962
Parent Cell Line:	HM-1 (ES Cell)
Strain of Origin:	129P2/OlaHsd-Hprt1b-m3

Allele Type:    Targeted (Null/knockout) Mutations:    Insertion, Intragenic deletion   Mutation details: The 3' end of the gene (exons 23-27) has been replaced with an HPRT minigene. In this allele, the more highly expressed DWM110 minigene was utilized. RT-PCR detected levels of Lig1-Hprt fusion transcripts although no protein product was detected by Western analysis. (J:75962)

View phenotypes and curated references for all genotypes (concatenated display).

Mouse strains and cell lines available from the International Mouse Strain Resource (IMSR)
Carrying this Mutation:	Mouse Strains: 0 strains available      Cell Lines: 0 lines available
Carrying any Lig1 Mutation:	48 strains or lines available

Original:	J:75962 Bentley DJ, et al., DNA ligase I null mouse cells show normal DNA repair activity but altered DNA replication and reduced genome stability. J Cell Sci. 2002 Apr 1;115(Pt 7):1551-61
All:	2 reference(s)