Mbnl2 RNA in situ Gene Expression Assay

Gene Expression Data
Assay Details

Assay

Reference:	J:153498 Diez-Roux G, et al., A high-resolution anatomical atlas of the transcriptome in the mouse embryo. PLoS Biol. 2011;9(1):e1000582
Assay type:	RNA in situ
MGI Accession ID:	MGI:4826118
Gene symbol:	Mbnl2
Gene name:	muscleblind like splicing factor 2

Probe:	IMAGE clone 875903
Probe preparation:	Antisense labelled with digoxigenin RNA
Visualized with:	Alkaline phosphatase
Assay notes:	Cryosections of fresh frozen material were fixed in 4% paraformaldehyde for 20 min. before further processing. A tyramide-biotin/streptavidin amplification step was included in the in situ hybridization procedure.

Results

Specimen euxassay_000081_07: embryonic day 14.5 (more

)

Structure	Level	Pattern	Image	Note
TS23: dorsal root ganglion	Strong	Regionally restricted	euxassay_000081_07

Specimen euxassay_000081_08: embryonic day 14.5 (more

)

Structure	Level	Pattern	Image	Note
TS23: dorsal root ganglion	Strong	Regionally restricted	euxassay_000081_08

Specimen euxassay_000081_09: embryonic day 14.5 (more

)

Structure	Level	Pattern	Image	Note
TS23: dorsal root ganglion	Moderate	Regionally restricted	euxassay_000081_09

Specimen euxassay_000081_11: embryonic day 14.5 (more

)
Note: Additional information for this specimen was included in the Eurexpress data submission file.

Structure	Level	Pattern	Image	Note
TS23: esophagus	Moderate	Regionally restricted	euxassay_000081_11	lumen

Specimen euxassay_000081_12: embryonic day 14.5 (more

)

Structure	Level	Pattern	Image	Note
TS23: esophagus	Moderate	Regionally restricted	euxassay_000081_12
TS23: midbrain	Strong	Graded	euxassay_000081_12

Specimen euxassay_000081_14: embryonic day 14.5 (more

)

Structure	Level	Pattern	Image
TS23: dorsal root ganglion	Strong	Regionally restricted	euxassay_000081_14
TS23: forebrain	Moderate	Graded	euxassay_000081_14
TS23: cerebral cortex	Moderate	Graded	euxassay_000081_14

Specimen euxassay_000081_15: embryonic day 14.5 (more

)

Structure	Level	Pattern	Image	Note
TS23: dorsal root ganglion	Strong	Uniform	euxassay_000081_15
TS23: trigeminal ganglion	Strong	Regionally restricted	euxassay_000081_15

Specimen euxassay_000081_16: embryonic day 14.5 (more

)

Structure	Level	Pattern	Image
TS23: vagus ganglion	Strong	Uniform	euxassay_000081_16
TS23: trigeminal ganglion	Strong	Uniform	euxassay_000081_16
TS23: glossopharyngeal ganglion	Strong	Uniform	euxassay_000081_16
TS23: dorsal root ganglion	Strong	Uniform	euxassay_000081_16

Specimen euxassay_000081_20: embryonic day 14.5 (more

)

Structure	Level	Pattern	Image	Note
TS23: trigeminal ganglion	Weak	Regionally restricted	euxassay_000081_20
TS23: lung	Weak	Spotted	euxassay_000081_20

Contributing Projects: Mouse Genome Database (MGD), Gene Expression Database (GXD), Mouse Models of Human Cancer database (MMHCdb) (formerly Mouse Tumor Biology (MTB)), Gene Ontology (GO)
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