Reporter:	lacZ
	Direct Detection
Assay notes:	Embryos were fixed in 0.2% glutaraldehyde, 2% formaldehyde, 2mM MgCl2 and 5mM EGTA.

Structure	Level	Pattern	Image	Note
TS6: primitive endoderm	Present		2A

Structure	Level	Pattern	Image	Note
TS8: extraembryonic visceral endoderm	Present		2B

Structure	Level	Pattern	Image	Note
TS7: extraembryonic visceral endoderm	Present		2C
TS7: parietal endoderm	Present		2C
TS7: primitive endoderm	Present		2C

Structure	Level	Pattern	Image	Note
TS7: extraembryonic visceral endoderm	Present		2D
TS7: parietal endoderm	Present		2D
TS7: primitive endoderm	Present		2D

Structure	Level	Pattern	Image	Note
TS11: extraembryonic visceral endoderm	Present	Regionally restricted	2E	Expression became restricted to the columnar visceral endoderm surrounding the proximal part of the egg cylinder.

Structure	Level	Pattern	Image	Note
TS11: definitive endoderm	Present	Regionally restricted	2F	Expression was detected in a few strongly positive cells flanking the node, which appeared to correspond to the emerging definitive endoderm.
TS11: node	Absent		2F
TS11: primitive streak	Absent		2F

Structure	Level	Pattern	Image	Note
TS12: embryo endoderm	Present	Regionally restricted	2G	Expression was detected in a few strongly positive cells flanking the node, which appeared to correspond to the emerging definitive endoderm.
TS12: primitive streak	Absent		2G	The node and the primitive streak were negative.

Structure	Level	Pattern	Image	Note
TS12: neural tube	Strong		2H

Structure	Level	Pattern	Image	Note
TS13: gut	Present	Regionally restricted	2I	Expression was detected in the gut epithelium.
TS13: neural tube	Strong	Uniform	2I

Structure	Level	Pattern	Image	Note
TS15: neural tube	Present	Regionally restricted	2J	Expression in the neural tube extended from the posterior border of the otic vesicle to the forelimb bud level.
TS15: foregut	Present	Regionally restricted	2J	Expression was detected in the dorsal foregut.
TS15: hindgut epithelium	Present		2J
TS15: mesenchyme derived from neural crest	Present		2J
TS15: midgut epithelium	Present		2J
TS15: spinal ganglion	Present		2J

Structure	Level	Pattern	Image	Note
TS15: neural tube	Present	Regionally restricted	2K	Expression was predominant in the ventral part and in the roof plate.
TS15: neural tube roof plate	Present		2K

Structure	Level	Pattern	Image	Note
TS16: foregut	Present	Regionally restricted	2L	Expression was detected in the dorsal foregut.
TS16: hindgut epithelium	Present		2L
TS16: liver	Present		2L
TS16: midgut epithelium	Present		2L
TS16: pancreas primordium	Present		2L

Structure	Level	Pattern	Image	Note
TS16: gut	Present		2M
TS16: pancreas primordium	Present		2M

Structure	Level	Pattern	Image	Note
TS15: mesonephric tubule	Present
TS15: nephric duct	Present

Structure	Level	Pattern	Image	Note
TS18: ureteric bud	Present	Regionally restricted		Expression was detected in the ureteric bud emerging from the most caudal portion of the Wolffian duct.

Structure	Level	Pattern	Image	Note
TS21: comma-shaped body	Strong		2N
TS21: s-shaped body	Present		2N

Structure	Level	Pattern	Image	Note
TS24: cortical renal tubule	Present	Regionally restricted	2O	Expression was detected in both the proximal and distal tubules.
TS24: cortical early proximal tubule	Present		2O
TS24: maturing glomerular tuft	Absent		2O

Structure	Level	Pattern	Image	Note
TS24: pancreas	Present		2P

Structure	Level	Pattern	Image	Note
TS7: conceptus	Present		4A

Structure	Level	Pattern	Image	Note
TS7: conceptus	Present		4B	Beta-galactosidase positive cells appeared rounded and loosely associated instead of forming a layer in close contact with neighbouring ectodermal cells.