Gene Expression Data
Assay Details
Assay
|
Reference:
|
J:122406
Moore-Scott BA, et al., Identification of molecular markers that are expressed in discrete anterior-posterior domains of the endoderm from the gastrula stage to mid-gestation. Dev Dyn. 2007 Jul;236(7):1997-2003
|
Assay type:
|
RNA in situ
|
MGI Accession ID:
|
MGI:3716119
|
Gene symbol:
|
Klf5
|
Gene name:
|
Kruppel-like transcription factor 5
|
Probe:
|
Klf5 cDNA1
|
Probe preparation:
|
Antisense labelled with digoxigenin RNA
|
Visualized with:
|
Alkaline phosphatase
|
Assay notes:
|
Embryos were post-fixed in 4% paraformaldehyde.
|
|
Results
|
Specimen
4A:
embryonic day 7.0
(more )
Note: Mid-gastrula.
Specimen
4A:
(close )
Genetic Background:
|
ICR
|
Age:
|
embryonic day 7.0
|
Age Note:
|
Age of embryo at noon of plug day not specified in reference.
|
Sex:
|
Not Specified
|
Type:
|
whole mount
|
Fixation:
|
Methanol
|
Note:
|
Mid-gastrula.
|
Structure |
Level |
Pattern |
Image |
Note |
TS10: primitive streak |
Present |
Regionally restricted |
4A
|
Expression is in the anterior primitive streak.
|
Specimen
4A':
embryonic day 7.0
(more )
Note: Mid-gastrula.
Specimen
4A':
(close )
Genetic Background:
|
ICR
|
Age:
|
embryonic day 7.0
|
Age Note:
|
Age of embryo at noon of plug day not specified in reference.
|
Sex:
|
Not Specified
|
Type:
|
section from whole mount
|
Fixation:
|
Methanol
|
Note:
|
Mid-gastrula.
|
Structure |
Level |
Pattern |
Image |
Note |
TS10: primitive streak |
Present |
Regionally restricted |
4A'
|
Expression is in the anterior primitive streak.
|
Specimen
4B:
embryonic day 7.5
(more )
Note: Late-gastrula.
Specimen
4B:
(close )
Genetic Background:
|
ICR
|
Age:
|
embryonic day 7.5
|
Age Note:
|
Age of embryo at noon of plug day not specified in reference.
|
Sex:
|
Not Specified
|
Type:
|
whole mount
|
Fixation:
|
Methanol
|
Note:
|
Late-gastrula.
|
Structure |
Level |
Pattern |
Image |
Note |
TS11: embryo ectoderm |
Present |
Regionally restricted |
4B
|
Expression is in the posterior endoderm and mesoderm/ ectoderm.
|
TS11: embryo endoderm |
Present |
Regionally restricted |
4B
|
Expression is in the posterior endoderm and mesoderm/ ectoderm.
|
TS11: embryo mesoderm |
Present |
Regionally restricted |
4B
|
Expression is in the posterior endoderm and mesoderm/ ectoderm.
|
Specimen
4B':
embryonic day 7.5
(more )
Note: Late-gastrula.
Specimen
4B':
(close )
Genetic Background:
|
ICR
|
Age:
|
embryonic day 7.5
|
Age Note:
|
Age of embryo at noon of plug day not specified in reference.
|
Sex:
|
Not Specified
|
Type:
|
section from whole mount
|
Fixation:
|
Methanol
|
Note:
|
Late-gastrula.
|
Structure |
Level |
Pattern |
Image |
Note |
TS11: embryo ectoderm |
Present |
Regionally restricted |
4B'
|
Expression is in the posterior endoderm and mesoderm/ ectoderm.
|
TS11: embryo endoderm |
Present |
Regionally restricted |
4B'
|
Expression is in the posterior endoderm and mesoderm/ ectoderm.
|
TS11: embryo mesoderm |
Present |
Regionally restricted |
4B'
|
Expression is in the posterior endoderm and mesoderm/ ectoderm.
|
Specimen
4C:
embryonic day 8.5
(more )
Specimen
4C:
(close )
Genetic Background:
|
ICR
|
Age:
|
embryonic day 8.5
|
Age Note:
|
Age of embryo at noon of plug day not specified in reference.
|
Sex:
|
Not Specified
|
Type:
|
whole mount
|
Fixation:
|
Methanol
|
Specimen
4D:
embryonic day 8.5
(more )
Specimen
4D:
(close )
Genetic Background:
|
ICR
|
Age:
|
embryonic day 8.5
|
Age Note:
|
Age of embryo at noon of plug day not specified in reference.
|
Sex:
|
Not Specified
|
Type:
|
whole mount
|
Fixation:
|
Methanol
|
Specimen
4D':
embryonic day 8.5
(more )
Specimen
4D':
(close )
Genetic Background:
|
ICR
|
Age:
|
embryonic day 8.5
|
Age Note:
|
Age of embryo at noon of plug day not specified in reference.
|
Sex:
|
Not Specified
|
Type:
|
section from whole mount
|
Fixation:
|
Methanol
|
Specimen
4E:
embryonic day 9.5
(more )
Specimen
4E:
(close )
Genetic Background:
|
ICR
|
Age:
|
embryonic day 9.5
|
Age Note:
|
Age of embryo at noon of plug day not specified in reference.
|
Sex:
|
Not Specified
|
Type:
|
whole mount
|
Fixation:
|
Methanol
|
Specimen
4E':
embryonic day 9.5
(more )
Specimen
4E':
(close )
Genetic Background:
|
ICR
|
Age:
|
embryonic day 9.5
|
Age Note:
|
Age of embryo at noon of plug day not specified in reference.
|
Sex:
|
Not Specified
|
Type:
|
section from whole mount
|
Specimen
4F:
embryonic day 11.0
(more )
Specimen
4F:
(close )
Genetic Background:
|
ICR
|
Age:
|
embryonic day 11.0
|
Age Note:
|
Age of embryo at noon of plug day not specified in reference.
|
Sex:
|
Not Specified
|
Type:
|
whole mount
|
Fixation:
|
Methanol
|
Specimen
4F':
embryonic day 11.0
(more )
Specimen
4F':
(close )
Genetic Background:
|
ICR
|
Age:
|
embryonic day 11.0
|
Age Note:
|
Age of embryo at noon of plug day not specified in reference.
|
Sex:
|
Not Specified
|
Type:
|
section from whole mount
|
Fixation:
|
Methanol
|
|