Probe:	Unc53H2 probe
Probe preparation:	Antisense RNA

Structure	Level	Pattern	Image	Note
TS13: 1st branchial arch ectoderm	Present		2A
TS13: 1st branchial arch mesenchyme	Present	Regionally restricted	2A	Expression is in the mesenchymal tissue immediately underlying the labelled epithelium in the maxillary region.

Structure	Level	Pattern	Image	Note
TS13: somite	Present	Regionally restricted	2B	Expression is in the inner surface of each dermomyotome in isolated cells.
TS13: future spinal cord neural plate	Present	Diffuse	2B
TS13: gut endoderm	Present	Diffuse	2B
TS13: sinus venosus	Present	Diffuse	2B
TS13: heart	Present	Regionally restricted	2B	Diffuse expression in the lining of the heart.

Structure	Level	Pattern	Image	Note
TS15: 1st branchial arch maxillary component	Present	Regionally restricted	2C	Expression is in the posterior part of region in a band that is continuous with the nasal placode.
TS15: 1st branchial arch mandibular component	Present	Regionally restricted	2C	Expression is anterior.
TS15: 1st branchial arch mandibular component ectoderm	Present	Regionally restricted	2C	Expression is in a proximo-distal bar of tissue midway between the anterior and posterior edges of the mandibular arch epithelium.
TS15: 1st branchial arch maxillary-mandibular groove ectoderm	Present		2C
TS15: 1st branchial groove	Present		2C
TS15: 2nd branchial groove	Present		2C
TS15: 3rd branchial groove	Present		2C
TS15: olfactory placode	Present		2C

Structure	Level	Pattern	Image	Note
TS15: head mesenchyme	Present	Regionally restricted	2D	Expression is in somites 1-4.
TS15: somite 05	Present		2D
TS15: somite 06	Present		2D
TS15: somite 07	Present		2D
TS15: somite 08	Present		2D
TS15: somite 09	Present		2D
TS15: somite 10	Present		2D
TS15: caudal neuropore	Weak		2D
TS15: gut	Weak		2D
TS15: heart	Weak		2D
TS15: tail mesenchyme	Weak		2D
TS15: otocyst epithelium	Strong	Regionally restricted	2D	Expression is in a small region in the center.
TS15: otocyst	Present	Regionally restricted	2D	Expression is weakly throughout and in a fine line in the prospective pigmented and neural retinal epithelium.

Structure	Level	Pattern	Image	Note
TS19: eye	Present	Regionally restricted	2E	Expression is in the closing developing eyelid.
TS19: branchial arch	Present	Regionally restricted	2E	Expression extends throughout the marginal epithelium and also to regions of the lateral epithelial surface.
TS19: forelimb bud ectoderm	Present		2E	Expression is stornger in the proximal epithelium at the anterior margin of the limb.
TS19: future leg ectoderm	Present		2E	Expression is stornger in the proximal epithelium at the anterior margin of the limb.
TS19: footplate ectoderm	Strong	Regionally restricted	2E	Expression is in a proximal region in the posterior margin of the plate.
TS19: handplate ectoderm	Strong	Regionally restricted	2E	Expression is in a proximal region in the posterior margin of the plate.
TS19: trunk somite	Present		2E	Expression is stongest in the somites at the level of the hindlimb.
TS19: tail somite	Strong		2E
TS19: genital tubercle	Present		2E
TS19: trunk mesenchyme	Present	Regionally restricted	2E	Expression is in a small, well-defined, oval area in the flank approximately midway between fore- and hind-limbs, which is observed on both sides.

Structure	Level	Pattern	Image	Note
TS23: cerebral cortex	Strong		3A
TS23: olfactory lobe	Strong		3A
TS23: dermis	Moderate		3A
TS23: medulla oblongata	Moderate		3A
TS23: midbrain	Moderate		3A

Structure	Level	Pattern	Image	Note
TS23: olfactory lobe	Strong		3B
TS23: medulla oblongata	Moderate		3B

Structure	Level	Pattern	Image	Note
TS23: skeletal muscle	Strong		3C	Shown is the head with occipital muscles.
TS23: cerebral cortex	Strong		3C
TS23: medulla oblongata	Moderate		3C

Structure	Level	Pattern	Image	Note
TS23: dorsal grey horn	Strong		3D
TS23: ventral grey horn	Strong		3D
TS23: dorsal root ganglion	Moderate		3D