Probe:	Glypican-4 cDNA1
Probe preparation:	Antisense labelled with digoxigenin RNA
Visualized with:	Alkaline phosphatase

Structure	Level	Pattern	Image	Note
TS11: embryo	Present	Regionally restricted	1A	Expression was detected in the anterior region.

Structure	Level	Pattern	Image	Note
TS11: mesendoderm	Present	Regionally restricted	1B	Expression was detected in anterior mesendoderm.
TS11: neural ectoderm	Absent		1B

Structure	Level	Pattern	Image	Note
TS12: neural ectoderm	Present		1C
TS12: future prosencephalon	Present	Regionally restricted	1C	Expression was most intense in the midline neuroepithelium of the forebrain.
TS12: embryo endoderm	Absent		1C

Structure	Level	Pattern	Image	Note
TS12: neural ectoderm	Present		1D
TS12: future prosencephalon	Present	Regionally restricted	1D	Expression was most intense in the midline neuroepithelium of the forebrain.
TS12: embryo endoderm	Absent		1D

Structure	Level	Pattern	Image	Note
TS12: neural ectoderm	Present		1E
TS12: embryo endoderm	Absent		1E

Structure	Level	Pattern	Image	Note
TS12: future prosencephalon	Present	Regionally restricted	1F	Expression expands laterally from midline.

Structure	Level	Pattern	Image	Note
TS12: future prosencephalon	Present	Regionally restricted	1G	Expression expands laterally from midline.

Structure	Level	Pattern	Image	Note
TS12: future prosencephalon	Present	Regionally restricted	1H	Expression expands laterally from midline.

Structure	Level	Pattern	Image	Note
TS12: future spinal cord neural plate	Present	Regionally restricted	1I	Expression was detected throughout the anterior neural ridge.
TS12: future prosencephalon	Present	Regionally restricted	1I	Expression was principally localized to the neuroepithelium of the anterior forebrain.
TS12: neural tube	Absent		1I	Expression was not detected in the neuroepithelium in the spinal region corresponding to the site of initiation of neural tube closure.

Structure	Level	Pattern	Image	Note
TS12: future spinal cord neural plate	Present	Regionally restricted	1J	Expression was detected throughout the anterior neural ridge.

Structure	Level	Pattern	Image	Note
TS12: future hindbrain neural crest	Weak		1K	Expression was detected at a more posterior level in the bending neural folds and in cells migrating lateral to the neural folds, which appear to be hindbrain neural crest.
TS12: future hindbrain neural fold	Weak		1K	Expression was detected at a more posterior level in the bending neural folds and in cells migrating lateral to the neural folds, which appear to be hindbrain neural crest.
TS12: migrating neural crest	Weak		1K	Expression was detected at a more posterior level in the bending neural folds and in cells migrating lateral to the neural folds, which appear to be hindbrain neural crest.

Structure	Level	Pattern	Image	Note
TS12: future spinal cord neural plate	Present	Regionally restricted	1L	Expression was detected throughout the anterior neural ridge.
TS12: future prosencephalon	Present	Regionally restricted	1L	Expression was principally localized to the neuroepithelium of the anterior forebrain.

Structure	Level	Pattern	Image	Note
TS13: future prosencephalon	Present	Regionally restricted	2A	Expression was detected in the neuroepithelium of ventral telencephalon, the rostral forebrain and the infundibular region of the ventral forebrain.
TS13: future spinal cord neural plate	Present	Regionally restricted	2A	Expression was detected in the anterior neural ridge.
TS13: neural tube	Absent		2A	Expression was not detected in the neuroepithelium in the spinal region corresponding to the site of initiation of neural tube closure.
TS13: neural ectoderm	Present	Regionally restricted	2A	Expression was detected in the neuroepithelium in the caudal region.
TS13: surface ectoderm	Present	Regionally restricted	2A	Expression was detected in the caudal region.
TS13: future hindbrain	Absent		2A
TS13: future midbrain	Absent		2A

Structure	Level	Pattern	Image	Note
TS13: future spinal cord neural plate	Present	Regionally restricted	2B	Expression was detected in the anterior neural ridge.
TS13: neural tube	Absent		2B	Expression was not detected in the neuroepithelium in the spinal region corresponding to the site of initiation of neural tube closure.
TS13: future prosencephalon	Present	Regionally restricted	2B	Expression was detected in the rostral forebrain and the infundibular region of the ventral forebrain.
TS13: future hindbrain	Absent		2B
TS13: future midbrain	Absent		2B

Structure	Level	Pattern	Image	Note
TS14: prosencephalon	Present	Regionally restricted	2E	Expression was detected in the neuroepithelium of ventral telencephalon.
TS14: central nervous system	Present	Regionally restricted	2E	Expression was detected in the neuroepithelium in the caudal region.
TS14: surface ectoderm	Present	Regionally restricted	2E	Expression was detected in the caudal region.

Structure	Level	Pattern	Image	Note
TS15: telencephalon	Strong	Uniform	2F	Expression was detected throughout the neuroepithelium of the entire telencephalon.
TS15: central nervous system	Present	Regionally restricted	2F	Expression was detected in the neuroepithelium in the caudal region.
TS15: surface ectoderm	Present	Regionally restricted	2F	Expression was detected in the caudal region.
TS15: 1st branchial arch	Present	Regionally restricted	2F	Expression was detected in the proximal region with lower intensity more distally.
TS15: 2nd branchial arch	Present	Regionally restricted	2F	Expression was detected in the proximal region with lower intensity more distally.
TS15: forelimb bud ectoderm	Present	Regionally restricted	2F	Expression was detected in a line in the surface ectoderm on the flank where the forelimb bud is forming.

Structure	Level	Pattern	Image	Note
TS14: future spinal cord neural fold	Present	Regionally restricted	2G	Expression was detected in the tips of neural folds.
TS14: surface ectoderm	Present		2G

Structure	Level	Pattern	Image	Note
TS14: prosencephalon	Present	Regionally restricted	2H	Expression was detected in the neuroepithelium of ventral telencephalon.

Structure	Level	Pattern	Image	Note
TS14: prosencephalon	Present	Regionally restricted	2I	Expression was detected in the neuroepithelium of ventral telencephalon.

Structure	Level	Pattern	Image	Note
TS14: central nervous system	Present	Regionally restricted	2J	Expression was detected in the neuroepithelium in the caudal region but was absent from the most ventral neuroepithelium.
TS14: surface ectoderm	Present	Regionally restricted	2J	Expression was detected in the caudal region.

Structure	Level	Pattern	Image	Note
TS16: telencephalon	Strong	Uniform	3A	Expression was detected throughout the neuroepithelium of the entire telencephalon.
TS16: central nervous system	Present	Regionally restricted	3A	Expression was detected in the neuroepithelium in the caudal region.
TS16: surface ectoderm	Present	Regionally restricted	3A	Expression was detected in the caudal region.
TS16: 3rd branchial arch	Present		3A
TS16: limb ectoderm	Present	Regionally restricted	3A	Expression was detected in the ectoderm on the ventral surface of the limb bud at the junction of the bud and the flank.
TS16: trunk somite	Present	Regionally restricted	3A	Expression was detected in a discrete stripe in the middle.

Structure	Level	Pattern	Image	Note
TS16: rhombomere	Present	Regionally restricted	3B	Expression was detected in the ventral domains of rhombomeres.

Structure	Level	Pattern	Image	Note
TS16: telencephalon	Strong	Uniform	3C	Expression was detected throughout the neuroepithelium of the entire telencephalon.
TS16: Rathke's pouch	Present		3C
TS16: infundibular recess of 3rd ventricle	Present	Regionally restricted	3C	Expression was detected in the infundibulum.
TS16: optic stalk	Present		3C
TS16: branchial arch ectoderm	Present	Regionally restricted	3C	Expression was detected on the external and internal surfaces of the branchial arch surface ectoderm.
TS16: oral epithelium	Present		3C	Expression was detected in the oral ectoderm.

Structure	Level	Pattern	Image	Note
TS15: otocyst	Present			Expression in the otic vesicles was first detected at E9.5.

Structure	Level	Pattern	Image	Note
TS16: otocyst	Present	Regionally restricted	3D	Expression was localized to the lateral half of each vesicle.

Structure	Level	Pattern	Image	Note
TS16: limb ectoderm	Present	Regionally restricted	3E	Expression was detected in the ectoderm on the ventral surface of the limb bud at the junction of the bud and the flank.
TS16: trunk somite	Present	Regionally restricted	3E	Expression was detected in the epaxial myotome - subjacent to the dermomyotome.

Structure	Level	Pattern	Image	Note
TS16: branchial arch ectoderm	Present	Regionally restricted	3F	Expression was detected on the external and internal surfaces of the branchial arch surface ectoderm.
TS16: oral epithelium	Present		3F	Expression was detected in the oral ectoderm.

Structure	Level	Pattern	Image	Note
TS16: optic cup inner layer	Present		3G	Expression was detected in the presumptive neuroretina.
TS16: optic cup outer layer	Absent		3G	Expression was absent in the presumptive retinal pigment epithelium.

Structure	Level	Pattern	Image	Note
TS16: Rathke's pouch	Present		3H
TS16: infundibular recess of 3rd ventricle	Present	Regionally restricted	3H	Expression was detected in the infundibulum.

Structure	Level	Pattern	Image	Note
TS16: central nervous system	Present	Regionally restricted	3I	Expression was detected in the neuroepithelium in the caudal region but was absent from the most ventral neuroepithelium.
TS16: surface ectoderm	Present	Regionally restricted	3I	Expression was detected in the caudal region but was absent in the midline.

Structure	Level	Pattern	Image	Note
TS17: telencephalon	Strong	Uniform	3J	Expression was detected throughout the neuroepithelium of the entire telencephalon.
TS17: central nervous system	Present	Regionally restricted	3J	Expression was detected in the neuroepithelium in the caudal region.
TS17: surface ectoderm	Present	Regionally restricted	3J	Expression was detected in the caudal region.
TS17: 3rd branchial arch	Present		3J
TS17: limb ectoderm	Present	Regionally restricted	3J	Expression was detected in the ectoderm on the ventral surface of the limb bud at the junction of the bud and the flank and the surface ectoderm on the dorsal aspect of the limb adjacent to the flank.
TS17: trunk somite	Present	Regionally restricted	3J	Expression was detected in a discrete stripe in the middle.
TS17: apical ectodermal ridge	Absent		3J

Structure	Level	Pattern	Image	Note
TS17: embryo	Present		3K

Structure	Level	Pattern	Image	Note
TS17: telencephalon	Strong	Uniform	3L	Expression was detected throughout the neuroepithelium of the entire telencephalon.

Structure	Level	Pattern	Image	Note
TS17: limb ectoderm	Present	Regionally restricted	3M	Expression was detected in the ectoderm on the ventral surface of the limb bud at the junction of the bud and the flank and the surface ectoderm on the dorsal aspect of the limb adjacent to the flank.

Structure	Level	Pattern	Image	Note
TS17: trunk somite	Present	Regionally restricted	3N	Expression was detected in the epaxial myotome - subjacent to the dermomyotome.

Structure	Level	Pattern	Image	Note
TS17: trunk somite	Present	Regionally restricted	3O	Expression was detected in a discrete stripe in the middle.

Structure	Level	Pattern	Image	Note
TS17: embryo	Present		3P

Structure	Level	Pattern	Image	Note
TS17: branchial arch ectoderm	Present	Regionally restricted	3Q	Expression was detected on the external and internal surfaces of the branchial arch surface ectoderm.
TS17: oral epithelium	Present		3Q	Expression was detected in the oral ectoderm.
TS17: 1st branchial arch mandibular component	Weak	Regionally restricted	3Q	Expression was weak or absent where the mandibular component of the first arch is beginning to fuse.

Structure	Level	Pattern	Image	Note
TS17: optic stalk	Present		3R
TS17: lens pit	Present		3R	Expression was detected in the lens placode.

Structure	Level	Pattern	Image	Note
TS17: eye	Present		3S

Structure	Level	Pattern	Image	Note
TS17: central nervous system	Present	Regionally restricted	3T	Expression was detected in the neuroepithelium in the caudal region but was absent from the most ventral neuroepithelium.
TS17: surface ectoderm	Present	Regionally restricted	3T	Expression was detected in the caudal region including the midline.