Gene Expression Data
Assay Details
|
Assay
|
|
Reference:
|
J:83932
Kablar B, et al., Myf5 and MyoD activation define independent myogenic compartments during embryonic development. Dev Biol. 2003 Jun 15;258(2):307-18
|
|
Assay type:
|
In situ reporter (knock in)
|
|
MGI Accession ID:
|
MGI:3531019
|
|
Gene symbol:
|
Myf5
|
|
Gene name:
|
myogenic factor 5
|
|
Reporter:
|
lacZ
|
|
|
Direct Detection
|
|
Assay notes:
|
The whole mounted embryos were fixed in 2% paraformaldehyde/0.2% glutaraldehyde.
|
|
|
Results
|
Specimen
4I:
embryonic day 10.5; Myf5tm1Pas/Myf5 +
(more  )
Specimen
4I:
(close  )
|
Genetic Background:
|
Not Specified
|
|
Mutant Allele(s):
|
Myf5tm1Pas/Myf5+
|
|
Age:
|
embryonic day 10.5
|
|
Age Note:
|
Age of embryo at noon of plug day not specified in reference.
|
|
Sex:
|
Not Specified
|
|
Type:
|
section
|
|
Embedding:
|
Paraffin
|
Specimen
4J:
(close )
|
Genetic Background:
|
involves: 129S4/SvJae
|
|
Mutant Allele(s):
|
Myf5tm1Pas/Myf5tm1Pas
Myod1tm1Jae/Myod1tm1Jae
|
|
Age:
|
embryonic day 10.5
|
|
Age Note:
|
Age of embryo at noon of plug day not specified in reference.
|
|
Sex:
|
Not Specified
|
|
Type:
|
section
|
|
Embedding:
|
Paraffin
|
Specimen
4K:
embryonic day 10.5; Myf5tm1Pas/Myf5 +
(more )
Specimen
4K:
(close )
|
Genetic Background:
|
Not Specified
|
|
Mutant Allele(s):
|
Myf5tm1Pas/Myf5+
|
|
Age:
|
embryonic day 10.5
|
|
Age Note:
|
Age of embryo at noon of plug day not specified in reference.
|
|
Sex:
|
Not Specified
|
|
Type:
|
section
|
|
Embedding:
|
Paraffin
|
Specimen
4L:
(close )
|
Genetic Background:
|
involves: 129S4/SvJae
|
|
Mutant Allele(s):
|
Myf5tm1Pas/Myf5tm1Pas
Myod1tm1Jae/Myod1tm1Jae
|
|
Age:
|
embryonic day 10.5
|
|
Age Note:
|
Age of embryo at noon of plug day not specified in reference.
|
|
Sex:
|
Not Specified
|
|
Type:
|
section
|
|
Embedding:
|
Paraffin
|
Specimen
5A:
embryonic day 11.5; Myf5tm1Pas/Myf5 +
(more )
Specimen
5A:
(close )
|
Genetic Background:
|
Not Specified
|
|
Mutant Allele(s):
|
Myf5tm1Pas/Myf5+
|
|
Age:
|
embryonic day 11.5
|
|
Age Note:
|
Age of embryo at noon of plug day not specified in reference.
|
|
Sex:
|
Not Specified
|
|
Type:
|
whole mount
|
|
Fixation:
|
Other - See Notes
|
Specimen
5B:
(close )
|
Genetic Background:
|
involves: 129S4/SvJae
|
|
Mutant Allele(s):
|
Myf5tm1Pas/Myf5tm1Pas
Myod1tm1Jae/Myod1tm1Jae
|
|
Age:
|
embryonic day 11.5
|
|
Age Note:
|
Age of embryo at noon of plug day not specified in reference.
|
|
Sex:
|
Not Specified
|
|
Type:
|
whole mount
|
|
Fixation:
|
Other - See Notes
|
| Structure |
Level |
Pattern |
Image |
Note |
| TS19: tail dermomyotome |
Present |
Regionally restricted |
5B
|
There is abherrent expression in the mutant. The expressing cells are retained outside of and adjacent to the dorsal and ventral dermomyotome lips.
|
| TS19: trunk dermomyotome |
Present |
Regionally restricted |
5B
|
There is abherrent expression in the mutant. The expressing cells are retained outside of and adjacent to the dorsal and ventral dermomyotome lips.
|
Specimen
5C:
embryonic day 12.5; Myf5tm1Pas/Myf5 +
(more )
Specimen
5C:
(close )
|
Genetic Background:
|
Not Specified
|
|
Mutant Allele(s):
|
Myf5tm1Pas/Myf5+
|
|
Age:
|
embryonic day 12.5
|
|
Age Note:
|
Age of embryo at noon of plug day not specified in reference.
|
|
Sex:
|
Not Specified
|
|
Type:
|
whole mount
|
|
Fixation:
|
Other - See Notes
|
Specimen
5D:
(close )
|
Genetic Background:
|
involves: 129S4/SvJae
|
|
Mutant Allele(s):
|
Myf5tm1Pas/Myf5tm1Pas
Myod1tm1Jae/Myod1tm1Jae
|
|
Age:
|
embryonic day 12.5
|
|
Age Note:
|
Age of embryo at noon of plug day not specified in reference.
|
|
Sex:
|
Not Specified
|
|
Type:
|
whole mount
|
|
Fixation:
|
Other - See Notes
|
| Structure |
Level |
Pattern |
Image |
Note |
| TS20: tail dermomyotome |
Present |
Regionally restricted |
5D
|
There is abherrent expression in the mutant. The expressing cells are retained outside of and adjacent to the dorsal and ventral dermomyotome lips.
|
| TS20: trunk mesenchyme |
Present |
Regionally restricted |
5D
|
There is abherrent expression in the mutant. The expressing cells are retained outside of and adjacent to the dorsal and ventral dermomyotome lips.
|
Specimen
5E:
embryonic day 13.5; Myf5tm1Pas/Myf5 +
(more )
Specimen
5E:
(close )
|
Genetic Background:
|
Not Specified
|
|
Mutant Allele(s):
|
Myf5tm1Pas/Myf5+
|
|
Age:
|
embryonic day 13.5
|
|
Age Note:
|
Age of embryo at noon of plug day not specified in reference.
|
|
Sex:
|
Not Specified
|
|
Type:
|
whole mount
|
|
Fixation:
|
Other - See Notes
|
Specimen
5F:
(close )
|
Genetic Background:
|
involves: 129S4/SvJae
|
|
Mutant Allele(s):
|
Myf5tm1Pas/Myf5tm1Pas
Myod1tm1Jae/Myod1tm1Jae
|
|
Age:
|
embryonic day 13.5
|
|
Age Note:
|
Age of embryo at noon of plug day not specified in reference.
|
|
Sex:
|
Not Specified
|
|
Type:
|
whole mount
|
|
Fixation:
|
Other - See Notes
|
Specimen
5G:
embryonic day 14.5; Myf5tm1Pas/Myf5 +
(more )
Specimen
5G:
(close )
|
Genetic Background:
|
Not Specified
|
|
Mutant Allele(s):
|
Myf5tm1Pas/Myf5+
|
|
Age:
|
embryonic day 14.5
|
|
Age Note:
|
Age of embryo at noon of plug day not specified in reference.
|
|
Sex:
|
Not Specified
|
|
Type:
|
whole mount
|
|
Fixation:
|
Other - See Notes
|
Specimen
5H:
(close )
|
Genetic Background:
|
involves: 129S4/SvJae
|
|
Mutant Allele(s):
|
Myf5tm1Pas/Myf5tm1Pas
Myod1tm1Jae/Myod1tm1Jae
|
|
Age:
|
embryonic day 14.5
|
|
Age Note:
|
Age of embryo at noon of plug day not specified in reference.
|
|
Sex:
|
Not Specified
|
|
Type:
|
whole mount
|
|
Fixation:
|
Other - See Notes
|
Specimen
5I:
embryonic day 15.5; Myf5tm1Pas/Myf5 +
(more )
Specimen
5I:
(close )
|
Genetic Background:
|
Not Specified
|
|
Mutant Allele(s):
|
Myf5tm1Pas/Myf5+
|
|
Age:
|
embryonic day 15.5
|
|
Age Note:
|
Age of embryo at noon of plug day not specified in reference.
|
|
Sex:
|
Not Specified
|
|
Type:
|
whole mount
|
|
Fixation:
|
Other - See Notes
|
Specimen
5J:
(close )
|
Genetic Background:
|
involves: 129S4/SvJae
|
|
Mutant Allele(s):
|
Myf5tm1Pas/Myf5tm1Pas
Myod1tm1Jae/Myod1tm1Jae
|
|
Age:
|
embryonic day 15.5
|
|
Age Note:
|
Age of embryo at noon of plug day not specified in reference.
|
|
Sex:
|
Not Specified
|
|
Type:
|
whole mount
|
|
Fixation:
|
Other - See Notes
|
Specimen
6A:
embryonic day 12.5; Myf5tm1Pas/Myf5 +
(more )
Note: This is a a transverse section.
Specimen
6A:
(close )
|
Genetic Background:
|
Not Specified
|
|
Mutant Allele(s):
|
Myf5tm1Pas/Myf5+
|
|
Age:
|
embryonic day 12.5
|
|
Age Note:
|
Age of embryo at noon of plug day not specified in reference.
|
|
Sex:
|
Not Specified
|
|
Type:
|
section
|
|
Embedding:
|
Paraffin
|
|
Note:
|
This is a a transverse section.
|
| Structure |
Level |
Pattern |
Image |
Note |
| TS20: trunk mesenchyme |
Present |
Regionally restricted |
6A
|
Expression is present in the myotome.
|
Specimen
6B:
(close )
|
Genetic Background:
|
involves: 129S4/SvJae
|
|
Mutant Allele(s):
|
Myf5tm1Pas/Myf5tm1Pas
Myod1tm1Jae/Myod1tm1Jae
|
|
Age:
|
embryonic day 12.5
|
|
Age Note:
|
Age of embryo at noon of plug day not specified in reference.
|
|
Sex:
|
Not Specified
|
|
Type:
|
section
|
|
Embedding:
|
Paraffin
|
|
Note:
|
This is a a transverse section.
|
| Structure |
Level |
Pattern |
Image |
Note |
| TS20: trunk mesenchyme |
Present |
Regionally restricted |
6B
|
Expression is present in cells that have delaminated from the myotome.
|
Specimen
6C:
embryonic day 12.5; Myf5tm1Pas/Myf5 +
(more )
Note: This is a a transverse section.
Specimen
6C:
(close )
|
Genetic Background:
|
Not Specified
|
|
Mutant Allele(s):
|
Myf5tm1Pas/Myf5+
|
|
Age:
|
embryonic day 12.5
|
|
Age Note:
|
Age of embryo at noon of plug day not specified in reference.
|
|
Sex:
|
Not Specified
|
|
Type:
|
section
|
|
Embedding:
|
Paraffin
|
|
Note:
|
This is a a transverse section.
|
Specimen
6D:
(close )
|
Genetic Background:
|
involves: 129S4/SvJae
|
|
Mutant Allele(s):
|
Myf5tm1Pas/Myf5tm1Pas
Myod1tm1Jae/Myod1tm1Jae
|
|
Age:
|
embryonic day 12.5
|
|
Age Note:
|
Age of embryo at noon of plug day not specified in reference.
|
|
Sex:
|
Not Specified
|
|
Type:
|
section
|
|
Embedding:
|
Paraffin
|
|
Note:
|
This is a a transverse section.
|
Specimen
6E:
embryonic day 12.5; Myf5tm1Pas/Myf5 +
(more )
Note: This is a a transverse section.
Specimen
6E:
(close )
|
Genetic Background:
|
Not Specified
|
|
Mutant Allele(s):
|
Myf5tm1Pas/Myf5+
|
|
Age:
|
embryonic day 12.5
|
|
Age Note:
|
Age of embryo at noon of plug day not specified in reference.
|
|
Sex:
|
Not Specified
|
|
Type:
|
section
|
|
Embedding:
|
Paraffin
|
|
Note:
|
This is a a transverse section.
|
Specimen
6F:
(close )
|
Genetic Background:
|
involves: 129S4/SvJae
|
|
Mutant Allele(s):
|
Myf5tm1Pas/Myf5tm1Pas
Myod1tm1Jae/Myod1tm1Jae
|
|
Age:
|
embryonic day 12.5
|
|
Age Note:
|
Age of embryo at noon of plug day not specified in reference.
|
|
Sex:
|
Not Specified
|
|
Type:
|
section
|
|
Embedding:
|
Paraffin
|
|
Note:
|
This is a a transverse section.
|
| Structure |
Level |
Pattern |
Image |
Note |
| TS20: tail dermomyotome |
Present |
Regionally restricted |
6F
|
Expression is present in cells that have delaminated from the myotome as well as cells in the dermotome.
|
| TS20: tail sclerotome |
Present |
Single cells |
6F
|
|
Specimen
7A:
embryonic day 14.5; Myf5tm1Pas/Myf5 +
(more )
Note: This is a a transverse section.
Specimen
7A:
(close )
|
Genetic Background:
|
Not Specified
|
|
Mutant Allele(s):
|
Myf5tm1Pas/Myf5+
|
|
Age:
|
embryonic day 14.5
|
|
Age Note:
|
Age of embryo at noon of plug day not specified in reference.
|
|
Sex:
|
Not Specified
|
|
Type:
|
section
|
|
Embedding:
|
Paraffin
|
|
Note:
|
This is a a transverse section.
|
Specimen
7B:
(close )
|
Genetic Background:
|
involves: 129S4/SvJae
|
|
Mutant Allele(s):
|
Myf5tm1Pas/Myf5tm1Pas
Myod1tm1Jae/Myod1tm1Jae
|
|
Age:
|
embryonic day 14.5
|
|
Age Note:
|
Age of embryo at noon of plug day not specified in reference.
|
|
Sex:
|
Not Specified
|
|
Type:
|
section
|
|
Embedding:
|
Paraffin
|
|
Note:
|
This is a a transverse section.
|
Specimen
7E:
embryonic day 14.5; Myf5tm1Pas/Myf5 +
(more )
Note: This is a a transverse section.
Specimen
7E:
(close )
|
Genetic Background:
|
Not Specified
|
|
Mutant Allele(s):
|
Myf5tm1Pas/Myf5+
|
|
Age:
|
embryonic day 14.5
|
|
Age Note:
|
Age of embryo at noon of plug day not specified in reference.
|
|
Sex:
|
Not Specified
|
|
Type:
|
section
|
|
Embedding:
|
Paraffin
|
|
Note:
|
This is a a transverse section.
|
Specimen
7F:
(close )
|
Genetic Background:
|
involves: 129S4/SvJae
|
|
Mutant Allele(s):
|
Myf5tm1Pas/Myf5tm1Pas
Myod1tm1Jae/Myod1tm1Jae
|
|
Age:
|
embryonic day 14.5
|
|
Age Note:
|
Age of embryo at noon of plug day not specified in reference.
|
|
Sex:
|
Not Specified
|
|
Type:
|
section
|
|
Embedding:
|
Paraffin
|
|
Note:
|
This is a a transverse section.
|
|
Analysis Tools