Probe:	Shh cDNA2
Probe preparation:	Antisense RNA

Structure	Level	Pattern	Image	Note
TS15: notochord	Present		3A	Expression is present along its entire length.
TS15: diencephalon floor plate	Present		3A	Expression is present along its entire length.
TS15: future hindbrain floor plate	Present		3A	Expression is present along its entire length.
TS15: future midbrain floor plate	Present		3A	Expression is present along its entire length.
TS15: neural tube floor plate	Present		3A	Expression is present along its entire length.
TS15: foregut	Present		3A
TS15: hindgut	Present		3A

Structure	Level	Pattern	Image	Note
TS15: notochord	Present		3B	Expression is present along its entire length.
TS15: diencephalon floor plate	Present		3B	Expression is present along its entire length.
TS15: future hindbrain floor plate	Present		3B	Expression is present along its entire length.
TS15: future midbrain floor plate	Present		3B	Expression is present along its entire length.
TS15: neural tube floor plate	Present		3B	Expression is present along its entire length.
TS15: foregut	Present		3B
TS15: hindgut	Present		3B

Structure	Level	Pattern	Image	Note
TS15: notochord	Present		3C	Expression is present along its entire length.
TS15: diencephalon floor plate	Present		3C	Expression is present along its entire length.
TS15: future hindbrain floor plate	Present		3C	Expression is present along its entire length.
TS15: future midbrain floor plate	Present		3C	Expression is present along its entire length.
TS15: neural tube floor plate	Present		3C	Expression is present along its entire length.
TS15: foregut	Present		3C
TS15: hindgut	Present		3C

Structure	Level	Pattern	Image	Note
TS15: hindgut	Present		3D
TS15: neural tube floor plate	Present		3D
TS15: notochord	Present		3D

Structure	Level	Pattern	Image	Note
TS15: neural tube floor plate	Present		3E	The floor plate is markedly broader in the mutant compared wild type.
TS15: notochord	Present		3E	The notochord is somewhat broader in the mutant compared wild type.
TS15: hindgut	Present		3E

Structure	Level	Pattern	Image	Note
TS17: hindbrain floor plate	Present			Expression remains excessively broad in the mutant along its entire length.
TS17: neural tube floor plate	Present			Expression remains excessively broad in the mutant along its entire length.

Structure	Level	Pattern	Image	Note
TS17: neural tube floor plate	Present			In the mutant, the floor plate has the same width as is seen in the wild type.

Structure	Level	Pattern	Image	Note
TS13: notochord	Present	Uniform	5A
TS13: primitive streak	Present	Uniform	5A

Structure	Level	Pattern	Image	Note
TS13: notochord	Present		5B	Expression is present throughout.
TS13: primitive streak	Present	Regionally restricted	5B	Expression is present in the rostral primitive streak.
TS13: foregut diverticulum endoderm	Present		5B
TS13: hindgut diverticulum endoderm	Present		5B

Structure	Level	Pattern	Image	Note
TS13: notochord	Present	Regionally restricted	5C	There is a longitudinal discontinuity of expression at the level of somites 4-5.

Structure	Level	Pattern	Image	Note
TS13: notochord	Present		5D
TS13: neural tube floor plate	Present		5D	Expression is being initiated at the ventral boundary of the neural plate, marking the onset of floor plate differentiation.

Structure	Level	Pattern	Image	Note
TS13: notochord	Present	Graded	5E	Expression is decreased at the midline in mutants relative to that seen in the wild type. At more rostral levels its expression is comparable to that seen in wild type.
TS13: neural tube floor plate	Present		5E	Expression is being initiated at the ventral boundary of the neural plate, marking the onset of floor plate differentiation.