Probe:	Daam2 cDNA4
Probe preparation:	Antisense labelled with digoxigenin RNA
Assay notes:	The authors did not specify which probe was used, either this probe or a shorter probe, Daam2 cDNA3 (MGI:3525597), was used.

Structure	Level	Pattern	Image	Note
TS10: embryo ectoderm	Weak

Structure	Level	Pattern	Image	Note
TS11: embryo mesoderm	Present	Regionally restricted	3A	Expression is in the proximal anterior mesoderm.
TS11: allantois	Strong	Regionally restricted	3A	Expression is at the base.

Structure	Level	Pattern	Image	Note
TS12: allantois	Present	Regionally restricted	3B	Expression is at the base.
TS12: head somite	Present	Regionally restricted	3B	Expression is in the newly-formed somites.
TS12: trunk somite	Present	Regionally restricted	3B	Expression is in the newly-formed somites.
TS12: trunk unsegmented mesenchyme	Present		3B

Structure	Level	Pattern	Image	Note
TS13: allantois	Present	Regionally restricted	3C	Expression is at the base of the allantois.
TS13: trunk somite	Present	Regionally restricted	3C	Expression is in the dermomyotome.
TS13: rhombomere 3	Present		3C
TS13: future midbrain	Present		3C
TS13: branchial arch	Present		3C

Structure	Level	Pattern	Image	Note
TS13: primitive streak	Present	Regionally restricted	3D	Expression is in the posterior part of the node.

Structure	Level	Pattern	Image	Note
TS14: rhombomere 3	Present		3E
TS14: rhombomere 5	Weak		3E
TS14: future midbrain	Strong	Spotted	3E

Structure	Level	Pattern	Image	Note
TS15: trunk dermomyotome	Present		3F
TS15: notochord	Strong	Regionally restricted	3F	Expression is at the chordoneural hinge at the posterior-most end of the notochord.
TS15: rhombomere 5	Strong		3F
TS15: rhombomere 3	Weak		3F
TS15: 1st branchial arch mandibular component mesenchyme	Present		3F
TS15: 3rd branchial arch	Present		3F
TS15: 2nd branchial arch	Present	Regionally restricted	3F	Expression is posterior.
TS15: visceral organ system	Present	Regionally restricted	3F	Expression is in the liver.
TS15: forelimb bud mesenchyme	Present	Single cells	3F
TS15: trunk mesenchyme	Present	Regionally restricted	3F	Expression is in the thickening of the lateral mesoderm wall that will become the hindlimb.
TS15: future midbrain	Absent		3F

Structure	Level	Pattern	Image	Note
TS14: rhombomere 5	Strong		3G
TS14: rhombomere 3	Weak		3G

Structure	Level	Pattern	Image	Note
TS17: tail somite	Present	Regionally restricted	3H	Expression is diffuse in anterior somites, but is expressed segmentally in posterior somites.
TS17: trunk somite	Present	Regionally restricted	3H	Expression is diffuse in anterior somites, but is expressed segmentally in posterior somites.
TS17: future spinal cord	Present		3H
TS17: hindbrain	Present		3H
TS17: 1st branchial arch mandibular component mesenchyme	Present		3H
TS17: 3rd branchial arch	Present		3H
TS17: 4th branchial arch	Present		3H
TS17: latero-nasal process	Present		3H
TS17: medial-nasal process	Present		3H
TS17: 2nd branchial arch	Present	Regionally restricted	3H	Expression is posterior.
TS17: forelimb bud mesenchyme	Present	Regionally restricted	3H	Expression is distal.
TS17: forebrain	Absent		3H
TS17: midbrain	Absent		3H

Structure	Level	Pattern	Image	Note
TS17: future spinal cord	Present		3I
TS17: hindbrain	Present		3I
TS17: forelimb bud mesenchyme	Present	Regionally restricted	3I	Expression is distal.
TS17: forebrain	Absent		3I
TS17: midbrain	Absent		3I

Structure	Level	Pattern	Image	Note
TS17: 4th ventricle	Present		3J
TS17: hindbrain	Present		3J
TS17: neural lumen	Present		3J
TS17: tail future spinal cord	Present	Regionally restricted	3J	Ventral expression.
TS17: forebrain	Absent		3J
TS17: midbrain	Absent		3J
TS17: latero-nasal process	Present	Regionally restricted	3J	Expression is strongest distally.
TS17: medial-nasal process	Present	Regionally restricted	3J	Expression is strongest distally.
TS17: midgut	Present	Regionally restricted	3J	Expression is in the mesenchyme.
TS17: genitourinary system	Present	Regionally restricted	3J	Expression is in the genital tubercle.

Structure	Level	Pattern	Image	Note
TS17: future spinal cord	Present		3K
TS17: hindbrain	Present		3K
TS17: tail future spinal cord	Present	Regionally restricted	3K	Ventral expression.
TS17: genitourinary system	Present	Regionally restricted	3K	Expression is in the genital tubercle.
TS17: forebrain	Absent		3K
TS17: midbrain	Absent		3K

Structure	Level	Pattern	Image	Note
TS17: future spinal cord	Present		3L
TS17: hindbrain	Present		3L
TS17: acoustic ganglion	Present		3L
TS17: forebrain	Absent		3L
TS17: midbrain	Absent		3L

Structure	Level	Pattern	Image	Note
TS17: future spinal cord	Present		3M
TS17: hindbrain	Present		3M
TS17: facial ganglion	Present		3M
TS17: trigeminal ganglion	Present		3M
TS17: forebrain	Absent		3M
TS17: midbrain	Absent		3M

Structure	Level	Pattern	Image	Note
TS20: tail	Present		3N
TS20: tail unsegmented mesenchyme	Present		3N
TS20: hindbrain	Present		3N
TS20: rib pre-cartilage condensation	Present		3N
TS20: spinal cord	Present		3N
TS20: vertebral cartilage condensation	Present		3N
TS20: forebrain	Absent		3N
TS20: midbrain	Absent		3N
TS20: head	Strong	Regionally restricted	3N	Expression is in the snout.
TS20: mandibular process	Strong		3N
TS20: maxillary process	Strong		3N
TS20: tongue	Strong		3N
TS20: vibrissa	Strong		3N
TS20: peripheral nervous system	Present	Regionally restricted	3N	Expression is in boundary cap cells.
TS20: forelimb digit	Present	Regionally restricted	3N	Expression is in the chondrogenic rays.
TS20: hindlimb digit	Present	Regionally restricted	3N	Expression is in the chondrogenic rays.

Structure	Level	Pattern	Image	Note
TS20: 4th ventricle	Present		3O
TS20: hindbrain	Present		3O
TS20: spinal cord	Present		3O
TS20: forebrain	Absent		3O
TS20: midbrain	Absent		3O

Structure	Level	Pattern	Image	Note
TS22: hindbrain	Present		3P
TS22: spinal cord	Present		3P
TS22: tongue	Present	Regionally restricted	3P	Expression is at the distal tip.
TS22: vibrissa	Present	Regionally restricted	3P	Expression is in the perimeter.
TS22: forebrain	Absent		3P
TS22: midbrain	Absent		3P

Structure	Level	Pattern	Image	Note
TS22: genital tubercle	Present		3Q
TS22: hindbrain	Present		3Q
TS22: spinal cord	Present		3Q
TS22: forebrain	Absent		3Q
TS22: midbrain	Absent		3Q

Structure	Level	Pattern	Image	Note
TS17: peripheral nervous system	Present	Regionally restricted	4B	Expression is in boundary cap cells.

Structure	Level	Pattern	Image	Note
TS17: future spinal cord	Present	Regionally restricted	4D	Expression is absent in the lateral spinal cord at more anterior positions in the embryo; it is present elsewhere.

Structure	Level	Pattern	Image	Note
TS17: lung mesenchyme	Strong		4F
TS17: lung epithelium	Absent		4F

Structure	Level	Pattern	Image	Note
TS17: stomach mesenchyme	Present	Patchy	4H

Structure	Level	Pattern	Image	Note
TS17: liver	Absent		4J

Structure	Level	Pattern	Image	Note
TS20: forelimb digit	Present	Regionally restricted	5C	Expression is in the chondrogenic rays.

Structure	Level	Pattern	Image	Note
TS22: forelimb digit 1 epidermis	Present	Regionally restricted	5D	Expression is at the distal tip.
TS22: forelimb digit 2 epidermis	Present	Regionally restricted	5D	Expression is at the distal tip.
TS22: forelimb digit 3 epidermis	Present	Regionally restricted	5D	Expression is at the distal tip.
TS22: forelimb digit 4 epidermis	Present	Regionally restricted	5D	Expression is at the distal tip.
TS22: forelimb digit 5 epidermis	Present	Regionally restricted	5D	Expression is at the distal tip.
TS22: forelimb digit	Present	Regionally restricted	5D	Expression is at the site of joint formation.
TS22: forelimb	Present	Regionally restricted	5D	Expression is at the lateral edges of developing tendons on the dorsal and ventral aspect of the forelimb and ventral wrist.