Antibody:

Anti-smooth muscle alpha-Actin (clone 1A4)

Structure	Level	Pattern	Image	Note
TS19: blood vessel	Strong	Uniform	6G , 6A , 6D	Expression is highlighted in the cranial vasculature.
TS19: dorsal aorta	Strong	Uniform	6G , 6A

Structure	Level	Pattern	Image	Note
TS19: blood vessel	Weak	Regionally restricted	6B , 6E	In this mutant, the vascular smooth muscle cells are absent in some vessels.
TS19: dorsal aorta	Weak		6B , 6H	In this mutant there are fewer vascular smooth muscle cells in the dorsal aorta.

Structure	Level	Pattern	Image	Note
TS19: blood vessel	Absent		6C , 6F	In this severely affected mutant, there are no vascular smooth muscle cells in the cranial vasculature.
TS19: dorsal aorta	Trace		6C , 6I	In this severely affected mutant there are few vascular smooth muscle cells in the dorsal aorta.

Structure	Level	Pattern	Image	Note
TS15: arterial system	Present
TS15: venous system	Present

Structure	Level	Pattern	Image	Note
TS15: arterial system	Weak			There are subtle differences in this mutant. Expression is generally decreased, especially in the cranial vasculature.
TS15: venous system	Weak			There are subtle differences in this mutant. Expression is generally decreased, especially in the cranial vasculature.

Structure	Level	Pattern	Image	Note
TS17: arterial system	Present
TS17: venous system	Present

Structure	Level	Pattern	Image	Note
TS17: arterial system	Weak			There are subtle differences in this mutant. Expression is generally decreased, especially in the cranial vasculature.
TS17: venous system	Weak			There are subtle differences in this mutant. Expression is generally decreased, especially in the cranial vasculature.